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Springer Protocols Handbooks

Experimental Protocols in Biotechnology

Editors: Gupta, Neelima, Gupta, Varsha (Eds.)

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  • Offers a broad range of experimental models and working standardized protocols
  • Supplemented with illustrations, figures and flow charts
  • Provides the latest evidence-based approaches to the diagnosis and management of haemoprotozoans and helminth infections
  • Details experimental protocols for the efficient quantification of micro RNAs and LncRNAs
  • Describes a plasmid vector-based technique for in-vivo protein visualization in live animals
  • Covers the principles, protocols, precautions and trouble-shooting for various biochemical techniques
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  • ISBN 978-1-0716-0607-0
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Hardcover $219.99
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  • ISBN 978-1-0716-0606-3
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About this book

This manual provides insights into the theory and practical aspects of several biotechnological and biochemical techniques for plants, protozoa, nematodes, insects and fishes, as well as human samples. The book also covers bioinformatics tools. The manual is an inclusive compilation, explaining techniques for microbial cultures, their diagnostics, DNA barcoding, microscopic techniques, blood analysis, parasite diagnostics through copro-antigens, enzyme analysis with enzyme kinetics, gene expression analysis, in-vivo protein visualization in live animals, geno-toxicity assays, quantification of micro RNAs and LncRNAs in tissue sections, the use of droplet PCR, and in-silico analysis. It provides step-by-step descriptions and details of each methodology, together with the final outcomes and inferences, in a simple and lucid manner, easily reproducible even for beginners. The broad range of techniques covered makes this volume extremely useful in understanding the principles of biotechniques, and simple applications for practical courses. 

               

About the authors

Prof. Neelima Gupta, M.Sc. Gold Medalist, M.Phil., Ph.D. in 1981 (Zoology) from the AMU, Aligarh, obtained her D.Sc. degree (Animal Science) from the MJP Rohilkhand University, Bareilly in 2012.  Presently working as Vice Chancellor of CSJM University, Kanpur, India since 2018.  Recipient of the Ek Janaki Ammal National Award for Animal Taxonomy (Ministry of Environment & Forests), Highest State Award, Saraswati Samman and Centre of Excellence (Government of UP), Vigyan Ratna Award (Council of Science & Technology, UP).  Has worked extensively on parasite taxonomy, aquatic toxicology of the Ramganga and Ganga Rivers, and fish health.  Nominated by the British Council, DST and INSA in various academic programs.


Dr. Varsha Gupta received her Ph.D (Life Sciences) from Jawaharlal Nehru University, New Delhi, in 2003. She then joined the National Centre for Plant Genome Research, New Delhi, for her post-doctoral training on plant genomics.  Presently working at Chhatrapati Shahu Ji Maharaj University, Kanpur since 2006.  She has done extensive work on molecular diagnostics using genomic and proteomic approaches, and has published several research articles and one textbook on “Basic and Applied Aspects of Biotechnology”.

              

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Enzyme-Linked Immunosorbent Assay Detection of Bacterial Wilt–Causing Ralstonia solanacearum

    Pages 1-18

    Umrao, Pramila Devi (et al.)

  • Diagnosis of Tropical Fascioliasis Using Specific Antigen Detection in Feces of Infected Human Being or Animal Species

    Pages 19-27

    Gupta, Neelima (et al.)

  • CMV Promoter-Driven Expression and Visualization of Tagged Proteins in Live and Fixed Zebrafish Embryonic Epidermis

    Pages 29-41

    Gupta, Kirti (et al.)

  • Detection of Blood Parasites and Estimation of Hematological Indices in Fish

    Pages 43-73

    Gupta, Neelima (et al.)

  • Techniques to Conduct Morphological and Molecular Investigations on Nematodes

    Pages 75-94

    Rana, Aasha (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-0716-0607-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-0606-3
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Experimental Protocols in Biotechnology
Editors
  • Neelima Gupta
  • Varsha Gupta
Series Title
Springer Protocols Handbooks
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0607-0
DOI
10.1007/978-1-0716-0607-0
Hardcover ISBN
978-1-0716-0606-3
Series ISSN
1949-2448
Edition Number
1
Number of Pages
XV, 246
Number of Illustrations
76 b/w illustrations, 3 illustrations in colour
Topics

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