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Group A Streptococcus

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Thomas Proft

    1. School of Medical Sciences, The University of Auckland, Auckland, New Zealand

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    You can also search for this editor in PubMed   Google Scholar

  2. Jacelyn M. S. Loh

    1. School of Medical Sciences, The University of Auckland, Auckland, New Zealand

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2136)

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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Genotyping and Genetic Manipulation of Group A Streptococcus

    1. Front Matter

      Pages 1-1
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    2. Detection of Streptococcus pyogenes Virulence Factors

      • Aleksandra KoziÅ„ska, Izabela Sitkiewicz
      Pages 3-16

    3. Closed-Tube Multiplex Real-Time PCR for the Detection of Group A Streptococcal Superantigens

      • Nour Zahi Gammoh, Lothar Rink
      Pages 17-23

    4. The emm-Cluster Typing System

      • Pierre R. Smeesters, Anne Botteaux
      Pages 25-31

    5. Protocols for Tn-seq Analyses in the Group A Streptococcus

      • Yoann Le Breton, Ashton T. Belew, Kevin S. McIver
      Pages 33-57

    6. Genetic Manipulation of Group A Streptococcus—Gene Deletion by Allelic Replacement

      • Timothy C. Barnett, Jessica N. Daw, Mark J. Walker, Stephan Brouwer
      Pages 59-69

    7. Generation of Bioluminescent Group A Streptococcus for Biophotonic Imaging

      • Jacelyn M. S. Loh, Kar Yan Soh, Thomas Proft
      Pages 71-77

  3. Application of Group A Streptococcus Omics

    1. Front Matter

      Pages 79-79
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    2. Whole Genome Sequence Analysis and Population Genomics of Group A Streptococci

      • Jake A. Lacey, Taylah B. James, Steven Y. C. Tong, Mark R. Davies
      Pages 81-111

    3. Group A Streptococcus Transcriptome Analysis

      • Misú Sanson, Anthony R. Flores
      Pages 113-133

    4. Protocol for Proteome Analysis of Group A Streptococcus

      • Laura Wilk
      Pages 135-144

    5. Investigation of Group A Streptococcal Interactions with Host Glycan Structures Using High-Throughput Techniques: Glycan Microarray Analysis Using Recombinant Protein and Whole Cells

      • Anuk Indraratna, David M. P. De Oliveira, Lauren E. Hartley-Tassell, Christopher J. Day, Mark J. Walker, Michael P. Jennings et al.
      Pages 145-151

  4. Assays to Investigate Group A Streptococcus Virulence Factors and Virulence Mechanisms

    1. Front Matter

      Pages 153-153
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    2. Using Lactococcus lactis as Surrogate Organism to Study Group A Streptococcus Surface Proteins

      • Catherine (Jia-Yun) Tsai, Jacelyn M. S. Loh, Sam Blanchett, Thomas Proft
      Pages 155-162

    3. Expression and Purification of Collagen-Like Proteins of Group A Streptococcus

      • Slawomir Lukomski, Dudley H. McNitt
      Pages 163-179

    4. Detection of Fibronectin-Binding Proteins of Streptococcus pyogenes Using Ligand Blot Analysis

      • Masanobu Nakata, Shigetada Kawabata
      Pages 181-190

    5. Morphology and Ultrastructure of Group A Streptococcus Biofilms

      • Tatyana A. Danilova, T. A. Smirnova, G. A. Danilina, A. A. Adzhieva, N. V. Shevlyagina, V. G. Zhukhovitsky
      Pages 191-198

    6. Dynamic Interactions of Group A Streptococcus with Host Macrophages

      • Angelica Montenegro Riestra, J. Andrés Valderrama
      Pages 199-222

    7. Identification of Group A Streptococcus-Containing Autophagosome-Like Vacuoles

      • Takashi Nozawa, Ichiro Nakagawa
      Pages 223-231
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Keywords

About this book

This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome analysis, application of GAS-derived molecular tools, and methods that are crucial for the evaluation of novel GAS vaccines and GAS virulence factors, including bactericidal assays and animal infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Group A Streptococcus: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • School of Medical Sciences, The University of Auckland, Auckland, New Zealand

    Thomas Proft, Jacelyn M. S. Loh

Bibliographic Information

  • Book Title: Group A Streptococcus

  • Book Subtitle: Methods and Protocols

  • Editors: Thomas Proft, Jacelyn M. S. Loh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0467-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0466-3Published: 20 May 2020

  • Softcover ISBN: 978-1-0716-0469-4Published: 20 May 2021

  • eBook ISBN: 978-1-0716-0467-0Published: 19 May 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 403

  • Number of Illustrations: 15 b/w illustrations, 39 illustrations in colour

  • Topics: Infectious Diseases, Virology

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