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Methods in Molecular Biology

Leptospira spp.

Methods and Protocols

Editors: Koizumi, Nobuo, Picardeau, Mathieu (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-0459-5
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About this book

This volume looks at all aspects of manipulation of Leptospira spp. from strain isolation to the latest techniques used to study the pathogenesis of leptospirosis. The chapters in this book cover topics such as the procedure to cultivate and isolate leptospires from both clinical and environmental samples; using methods like whole genome sequencing and Matrix Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry to identify bacterial species; tools for gene inactivation and in vitro and in vivo assays to study the pathogenesis of leptospirosis; and the use of hamsters to evaluate leptospiral virulence and vaccine candidates. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Leptospira spp.: Methods and Protocols is a valuable resource for researchers interested in learning more about this developing field and these fascinating organisms.  


Table of contents (25 chapters)

Table of contents (25 chapters)
  • Isolation and Culture of Leptospira from Clinical and Environmental Samples

    Pages 1-9

    Goarant, Cyrille (et al.)

  • Core Genome Multi-locus Sequence Typing Analyses of Leptospira spp. Using the Bacterial Isolate Genome Sequence Database

    Pages 11-21

    Grillová, Linda (et al.)

  • Use of MALDI-ToF Mass Spectrometry for Identification of Leptospira

    Pages 23-29

    Girault, Dominique (et al.)

  • Cell Enumeration of Leptospira by Flow Cytometry

    Pages 31-40

    Fontana, Célia (et al.)

  • The Single-Step Method of RNA Purification Applied to Leptospira

    Pages 41-51

    Zavala-Alvarado, Crispin (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-0716-0459-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-0458-8
  • with online files
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Leptospira spp.
Book Subtitle
Methods and Protocols
Editors
  • Nobuo Koizumi
  • Mathieu Picardeau
Series Title
Methods in Molecular Biology
Series Volume
2134
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0459-5
DOI
10.1007/978-1-0716-0459-5
Hardcover ISBN
978-1-0716-0458-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 291
Number of Illustrations
10 b/w illustrations, 56 illustrations in colour
Topics

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