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Animal Models of Diabetes

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2128)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. Practical Considerations when Using Mouse Models of Diabetes

    • Aileen J. F. King, Lydia F. Daniels Gatward, Matilda R. Kennard
    Pages 1-10
  3. Selectively Bred Diabetes Models: GK Rats, NSY Mice, and ON Mice

    • Mototsugu Nagao, Jonathan Lou S. Esguerra, Anna Wendt, Akira Asai, Hitoshi Sugihara, Shinichi Oikawa et al.
    Pages 25-54
  4. A Review of Mouse Models of Monogenic Diabetes and ER Stress Signaling

    • Paraskevi Salpea, Cristina Cosentino, Mariana Igoillo-Esteve
    Pages 55-67
  5. Rat Models of Human Type 1 Diabetes

    • Sigurd Lenzen, Tanja Arndt, Matthias Elsner, Dirk Wedekind, Anne Jörns
    Pages 69-85
  6. Mouse Models of Autoimmune Diabetes: The Nonobese Diabetic (NOD) Mouse

    • Dawei Chen, Terri C. Thayer, Li Wen, F. Susan Wong
    Pages 87-92
  7. Mouse Models of Virus-Induced Type 1 Diabetes

    • Gustaf Christoffersson, Malin Flodström-Tullberg
    Pages 93-105
  8. Rat Models of Virus-Induced Type 1 Diabetes

    • James C. Needell, Danny Zipris
    Pages 107-114
  9. Large Animal Models of Diabetes

    • Barbara Ludwig, Eckhard Wolf, Uwe Schönmann, Stefan Ludwig
    Pages 115-134
  10. Use of Streptozotocin in Rodent Models of Islet Transplantation

    • Aileen J. F. King, Elisabet Estil·les, Eduard Montanya
    Pages 135-147
  11. Generating Beta-Cell-Specific Transgenic Mice Using the Cre-Lox System

    • Lorna I. F. Smith, Thomas G. Hill, James E. Bowe
    Pages 181-205
  12. The Glucose Tolerance Test in Mice

    • Patricia Fonseca Pedro, Anastasia Tsakmaki, Gavin A. Bewick
    Pages 207-216
  13. Assessment of Insulin Tolerance In Vivo in Mice

    • Irene Cózar-Castellano, Germán Perdomo
    Pages 217-224
  14. Continuous Glucose Monitoring in Conscious Unrestrained Mice

    • Aileen J. F. King, Matilda R. Kennard, Manasi Nandi
    Pages 225-239
  15. Assessing Mouse Islet Function

    • Patricio Atanes, Inmaculada Ruz-Maldonado, Oladapo E. Olaniru, Shanta J. Persaud
    Pages 241-268
  16. Assessing Immune Responses in the Nonobese Diabetic Mouse Model of Type 1 Diabetes

    • Terri C. Thayer, Dimitri Kakabadse, Joanne Boldison, F. Susan Wong
    Pages 269-289
  17. Assessment of Insulin Tolerance Ex Vivo

    • Irene Cózar-Castellano, Germán Perdomo
    Pages 291-300

About this book

This volume discusses a variety of animal models of diabetes, as well as describes techniques used to study end-points when using these models. The chapters in this book cover topics such as important considerations when working with mouse models of diabetes, highlighting factors that new investigators may not be aware of and some potential pitfalls in experimental outcomes; main characteristics of some commonly used animal models of diabetes research, ranging from mice to primates; animal models used to study specific aspects of beta-cell biology; and a focus on techniques used to assess blood glucose homeostasis, insulin action, and islet function in vivo and ex vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and comprehensive, Animal Models of Diabetes: Methods and Protocols is a valuable resource that will help diabetes researchers design and carry out in vivo studies that will best suit their experimental questions and needs.  


Editors and Affiliations

  • Department of Diabetes, School of Life Course Sciences, King’s College London, London, UK

    Aileen J. F. King

Bibliographic Information

  • Book Title: Animal Models of Diabetes

  • Book Subtitle: Methods and Protocols

  • Editors: Aileen J. F. King

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0385-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0384-0Published: 17 March 2020

  • Softcover ISBN: 978-1-0716-0387-1Published: 17 March 2020

  • eBook ISBN: 978-1-0716-0385-7Published: 16 March 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 342

  • Number of Illustrations: 10 b/w illustrations, 39 illustrations in colour

  • Topics: Molecular Medicine, Animal Models

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access