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Methods in Molecular Biology
cover

Expression, Purification, and Structural Biology of Membrane Proteins

Editors: Perez, Camilo, Maier, Timm (Eds.)

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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eBook $169.00
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  • ISBN 978-1-07-160373-4
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Hardcover $219.99
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  • ISBN 978-1-0716-0372-7
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About this book

This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 
Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins.
Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.

Table of contents (25 chapters)

Table of contents (25 chapters)
  • Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae

    Pages 1-11

    Shaw, Porsha L. R. (et al.)

  • Membrane Protein Production in Escherichia coli

    Pages 13-27

    McIlwain, Benjamin C. (et al.)

  • Membrane Protein Production in Lactococcus lactis for Structural Studies

    Pages 29-45

    Martens, Chloe

  • Expression and Purification of Membrane Proteins in Saccharomyces cerevisiae

    Pages 47-61

    King, Martin S. (et al.)

  • Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System

    Pages 63-80

    Boivineau, Jacques (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-07-160373-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-0716-0372-7
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Expression, Purification, and Structural Biology of Membrane Proteins
Editors
  • Camilo Perez
  • Timm Maier
Series Title
Methods in Molecular Biology
Series Volume
2127
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160373-4
DOI
10.1007/978-1-0716-0373-4
Hardcover ISBN
978-1-0716-0372-7
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 423
Number of Illustrations
11 b/w illustrations, 78 illustrations in colour
Topics

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