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Biolistic DNA Delivery in Plants

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Sachin Rustgi

    1. Department of Plant and Environmental Sciences, School of Health Research, Pee Dee Research and Education Center, Clemson University, Florence, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Hong Luo

    1. Department of Genetics and Biochemistry, Clemson University, Clemson, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2124)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Tribute to Professor Diter von Wettstein

    1. Front Matter

      Pages 1-1
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    2. Diter von Wettstein, Professor of Genetics and Master of Translating Science into Applications

      • Sachin Rustgi, Birgitte Skadhauge
      Pages 3-18

    3. Diter von Wettstein and The Meiotic Program of Pairing and Recombination

      • Denise Zickler
      Pages 19-35

  3. Background and Overview

    1. Front Matter

      Pages 37-37
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    2. A Short History and Perspectives on Plant Genetic Transformation

      • Thakku R. Ramkumar, Sangram K. Lenka, Sagar S. Arya, Kailash C. Bansal
      Pages 39-68

    3. Choice of the Promoter for Tissue and Developmental Stage-Specific Gene Expression

      • Olga G. Smirnova, Alex V. Kochetov
      Pages 69-106

    4. Choice of Explant for Plant Genetic Transformation

      • Nibedita Chakraborty, Priyanka Chakraborty, Moutushi Sen, Rajib Bandopadhyay
      Pages 107-123

    5. Biolistic Approach for Transient Gene Expression Studies in Plants

      • Benoît Lacroix, Vitaly Citovsky
      Pages 125-139

    6. Nanobiolistics: An Emerging Genetic Transformation Approach

      • Francis J. Cunningham, Gozde S. Demirer, Natalie S. Goh, Huan Zhang, Markita P. Landry
      Pages 141-159

  4. Protocols

    1. Front Matter

      Pages 161-161
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    2. Biolistic Transformation of Japonica Rice Varieties

      • Raviraj Banakar, Kan Wang
      Pages 163-176

    3. Biolistic DNA Delivery in Maize Immature Embryos

      • Kan Wang, Huilan Zhu, Morgan McCaw
      Pages 177-195

    4. Biolistic DNA Delivery and Its Applications in Sorghum bicolor

      • Guoquan Liu, Karen Massel, Basam Tabet, Ian D. Godwin
      Pages 197-215

    5. Biolistics-Mediated Gene Delivery in Sugarcane

      • Priya A. Joyce, Yue Sun
      Pages 217-228

    6. Genetic Transformation of Common Wheat (Triticum aestivum L.) Using Biolistics

      • Caroline A. Sparks, Angela Doherty
      Pages 229-250

    7. Biolistic DNA Delivery in Turfgrass Embryonic Callus Initiated from Mature Seeds

      • Man Zhou, Junming Zhao, Dayong Li, Shuangrong Yuan, Ning Yuan, Zhigang Li et al.
      Pages 251-261

    8. Use of Microspore-Derived Calli as Explants for Biolistic Transformation of Common Wheat

      • Sachin Rustgi, Samneet Kashyap, Nii Ankrah, Diter von Wettstein
      Pages 263-279

    9. Plant Transformation Techniques: Agrobacterium- and Microparticle-Mediated Gene Transfer in Cereal Plants

      • Jafargholi Imani, Karl-Heinz Kogel
      Pages 281-294

    10. Proteolistics: A Protein Delivery Method

      • Susana Martin-Ortigosa, Kan Wang
      Pages 295-307
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Keywords

About this book

This volume details protocols for the use of the biolistic DNA delivery method in different plant species. Chapters guide readers through non-protocol chapters that cover relevant topics of interest, a broad overview of the field, exciting modifications of the system, and reliable plant transformation procedures in different plant species.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Biolistic DNA Delivery: Methods and Protocols aims to provide a comprehensive collection of protocols to intended to be a practical guide for the novice as well as the advanced user in the field of plant genetic transformation.

Editors and Affiliations

  • Department of Plant and Environmental Sciences, School of Health Research, Pee Dee Research and Education Center, Clemson University, Florence, USA

    Sachin Rustgi

  • Department of Genetics and Biochemistry, Clemson University, Clemson, USA

    Hong Luo

Bibliographic Information

  • Book Title: Biolistic DNA Delivery in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Sachin Rustgi, Hong Luo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0356-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0355-0Published: 11 April 2020

  • Softcover ISBN: 978-1-0716-0358-1Published: 11 April 2021

  • eBook ISBN: 978-1-0716-0356-7Published: 10 April 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 334

  • Number of Illustrations: 2 b/w illustrations, 35 illustrations in colour

  • Topics: Plant Genetics and Genomics

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