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Animal Influenza Virus

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Erica Spackman

    1. Exotic and Emerging Avian Viral Diseases Unit, US National Poultry Research Center, US Department of Agriculture, Agricultural Research Service, Athens, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2123)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. General

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Hemagglutination Assay for Influenza Virus

      • Mary Lea Killian
      Pages 3-10

    3. Hemagglutination Inhibition Assay

      • Erica Spackman, Ioannis Sitaras
      Pages 11-28

    4. Immunohistochemical Staining of Influenza Virus in Tissues

      • Mary J. Pantin-Jackwood
      Pages 29-36

    5. Plasmid-Based Reverse Genetics of Influenza A Virus

      • Daniel R. Perez, Brittany Seibert, Lucas Ferreri, Chang-Won Lee, Daniela Rajao
      Pages 37-59

    6. Antigenic Cartography: Overview and Current Developments

      • Ioannis Sitaras
      Pages 61-68

    7. Complete Genome Sequencing of Influenza A Viruses Using Next-Generation Sequencing

      • Dong-Hun Lee
      Pages 69-79

  3. Avian Influenza

    1. Front Matter

      Pages 81-81
      Download chapter PDF

    2. A Brief Introduction to Avian Influenza Virus

      • Erica Spackman
      Pages 83-92

    3. Wild Bird Surveillance for Avian Influenza Virus

      • Rebecca L. Poulson, Justin D. Brown
      Pages 93-112

    4. Avian Influenza Virus Sample Types, Collection, and Handling

      • Mary Lea Killian
      Pages 113-121

    5. Avian Influenza Virus RNA Extraction

      • Erica Spackman, Scott A. Lee
      Pages 123-135

    6. Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR

      • Erica Spackman
      Pages 137-148

    7. Avian Influenza Virus Isolation, Propagation, and Titration in Embryonated Chicken Eggs

      • Erica Spackman, Mary Lea Killian
      Pages 149-164

    8. Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus

      • Terra A. Jenson
      Pages 165-175

    9. Detection of Influenza A Antibodies in Avian Samples by ELISA

      • Erica Spackman, Mary Lea Killian
      Pages 177-193

    10. Conducting Influenza Virus Pathogenesis Studies in Avian Species

      • Carol J. Cardona, David A. Halvorson, Jeffrey Hall, Mary J. Pantin-Jackwood, Justin D. Brown
      Pages 195-209

    11. Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry

      • David E. Swayne
      Pages 211-225

    12. Techniques for the Measurement of Cell Mediated Immune Responses to Avian Influenza Virus

      • Darrell R. Kapczynski, Karen Segovia
      Pages 227-245
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Keywords

About this book

This third edition aims to provide new and updated methods on animal influenza viruses as well as more advanced protocols that will guide the reader in designing research. Chapters detail influenza in peridomestic animals, marine mammals, savian influenza, swine influenza, equine influenza, hemagglutination,  genome sequencing, and influenza in other mammals. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Animal Influenza Virus: Methods and Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

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Editors and Affiliations

  • Exotic and Emerging Avian Viral Diseases Unit, US National Poultry Research Center, US Department of Agriculture, Agricultural Research Service, Athens, USA

    Erica Spackman

Bibliographic Information

  • Book Title: Animal Influenza Virus

  • Book Subtitle: Methods and Protocols

  • Editors: Erica Spackman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0346-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0345-1Published: 14 March 2020

  • Softcover ISBN: 978-1-0716-0348-2Published: 14 March 2020

  • eBook ISBN: 978-1-0716-0346-8Published: 13 March 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XII, 454

  • Number of Illustrations: 10 b/w illustrations, 46 illustrations in colour

  • Topics: Infectious Diseases

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