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Methods in Molecular Biology

DNA Electrophoresis

Methods and Protocols

Editors: Hanada, Katsuhiro (Ed.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-07-160323-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-07-160322-2
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field.


Table of contents (18 chapters)

Table of contents (18 chapters)
  • Introduction and Perspectives of DNA Electrophoresis

    Pages 1-13

    Hanada, Katsuhiro

  • Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers

    Pages 15-24

    Gibson, Elizabeth G. (et al.)

  • Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage

    Pages 25-42

    Kolesar, Jill E. (et al.)

  • 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast

    Pages 43-59

    Zardoni, Luca (et al.)

  • Neutral–Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures

    Pages 61-72

    Mettrick, Karla A. (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-07-160323-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-07-160322-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
DNA Electrophoresis
Book Subtitle
Methods and Protocols
Editors
  • Katsuhiro Hanada
Series Title
Methods in Molecular Biology
Series Volume
2119
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160323-9
DOI
10.1007/978-1-0716-0323-9
Hardcover ISBN
978-1-07-160322-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 228
Number of Illustrations
30 b/w illustrations, 40 illustrations in colour
Topics