Editors:
- includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2117)
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Table of contents (19 protocols)
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Front Matter
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Next-Generation Sequencing Technologies and Data Analysis
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Front Matter
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Analysis and Visualization of Single-Cell and Bulk RNA-Seq Transcriptome Data
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Front Matter
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Transcriptional Programs that Promote Reprogramming, Transdifferentiation, and Cancer Formation
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Front Matter
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About this book
This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Stem Cell Transcriptional Network: Methods and Protocols, Second Edition aims to provide a key resource for biologists seeking to interrogate these vital networks.
Editors and Affiliations
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Karmanos Cancer Institute, Wayne State University School of Medicine, Detroit, USA
Benjamin L. Kidder
Bibliographic Information
Book Title: Stem Cell Transcriptional Networks
Book Subtitle: Methods and Protocols
Editors: Benjamin L. Kidder
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0301-7
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0300-0Published: 21 January 2020
Softcover ISBN: 978-1-0716-0303-1Published: 21 January 2021
eBook ISBN: 978-1-0716-0301-7Published: 20 January 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 308
Number of Illustrations: 8 b/w illustrations, 67 illustrations in colour
Topics: Stem Cells