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Methods in Molecular Biology

RNA Spectroscopy

Methods and Protocols

Editors: Arluison, Véronique, Wien, Frank (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-07-160278-2
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  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-07-160277-5
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  • Usually dispatched within 3 to 5 business days.
About this book

This volume looks at the different spectroscopic and biophysical methods used by researchers to study the structure and folding of RNA, and to follow their interactions with proteins. The chapters in this book cover topics such as single-molecule spectroscopy of multiple RNA species; surface plasmon resonance, MS or microcalorimetry for investigating molecular interactions with RNA; FTIR, SAXS, SANS and SRCD spectroscopies to analyze RNA structure; use of fluorescent nucleotides to map RNA-binding sites on proteins surfaces or CryoEM; and much more. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, RNA Spectroscopy: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.


Table of contents (22 chapters)

Table of contents (22 chapters)
  • Encapsulation of Fluorescently Labeled RNAs into Surface-Tethered Vesicles for Single-Molecule FRET Studies in TIRF Microscopy

    Pages 1-16

    Zelger-Paulus, Susann (et al.)

  • Preparation of SNAPf-Beads for Colocalization Single-Molecule Spectroscopy (CoSMoS) of RNA-Protein Complexes

    Pages 17-22

    Haraszti, Reka A. (et al.)

  • Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species

    Pages 23-29

    Haraszti, Reka A. (et al.)

  • Dynamic Light Scattering Analysis on RNA Associated to Proteins

    Pages 31-39

    Bernacchi, Serena

  • Bioinformatic Application of Fluorescence-Based In Vivo RNA Regional Accessibility Data to Identify Novel sRNA Targets

    Pages 41-71

    Bowman, Emily K. (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-07-160278-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-07-160277-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNA Spectroscopy
Book Subtitle
Methods and Protocols
Editors
  • Véronique Arluison
  • Frank Wien
Series Title
Methods in Molecular Biology
Series Volume
2113
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160278-2
DOI
10.1007/978-1-0716-0278-2
Hardcover ISBN
978-1-07-160277-5
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 356
Number of Illustrations
16 b/w illustrations, 80 illustrations in colour
Topics