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Methods in Molecular Biology

T-Cell Receptor Signaling

Methods and Protocols

Editors: Liu, Chaohong (Ed.)

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  • Includes cutting-edge methods and protocols 
  • Provides step-by-step detail essential for reproducible results 
  • Contains key notes and implementation advice from the experts
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eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-07-160266-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-07-160265-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This volume provides current and new advanced methods and protocols to study T cells. Chapters guide readers through T cell diversity using mass cytometry, analyzing T cells from single cell level, CRISPR/Cas9 techniques to study the T cell activation, techniques to study subsets of Tcell’s, procedures to study artificial antigen presentosomes for T cell activation, techniques to study the T cell development, two-photon microscopy, and MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, T-Cell Receptor Signaling: Methods and Protocols aims to provide a wide range of approaches and be an invaluable resource for present and future generations of T cell researchers.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Exploration of T-Cell Diversity Using Mass Cytometry

    Pages 1-20

    O’Boyle, Kaitlin C. (et al.)

  • A Carrier Strategy for Mass Cytometry Analysis of Small Numbers of Cells

    Pages 21-33

    Jia, Xian (et al.)

  • Simultaneous Measurement of Surface Proteins and Gene Expression from Single Cells

    Pages 35-46

    Luo, Jiadi (et al.)

  • Analysis of Transcriptional Profiling of Immune Cells at the Single-Cell Level

    Pages 47-57

    Ferguson, Annabel (et al.)

  • CRISPR/Cas9-Based Genetic Screening to Study T-Cell Function

    Pages 59-70

    Shang, Wanjing (et al.)

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-07-160266-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-1-07-160265-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
T-Cell Receptor Signaling
Book Subtitle
Methods and Protocols
Editors
  • Chaohong Liu
Series Title
Methods in Molecular Biology
Series Volume
2111
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160266-9
DOI
10.1007/978-1-0716-0266-9
Hardcover ISBN
978-1-07-160265-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XII, 296
Number of Illustrations
16 b/w illustrations, 48 illustrations in colour
Topics