Methods in Molecular Biology

RNA Chaperones

Methods and Protocols

Editors: Heise, Tilman (Ed.)

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts
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  • ISBN 978-1-07-160231-7
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Hardcover $219.99
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About this book

This book provides a wide spectrum of methods to study RNA chaperones in vitro, at the single molecule level, and protocols useful for cell-based assays. Beginning with a section on a number of bacterial proteins for study, the volume also explores proteins from eukaryotic cells and how to delve into the complex interactions between RNA chaperones and the folding and unfolding of proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, RNA Chaperones: Methods and Protocols serves as an ideal guide for scientists and students interested in RNA biology and RNA chaperones.

Chapter 3 is available Open Access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones

    Pages 1-18

    Kim, Hyeong Jin (et al.)

  • Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy

    Pages 19-39

    Panja, Subrata (et al.)

  • Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity

    Pages 41-58

    Menendez-Gil, Pilar (et al.)

  • Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding

    Pages 59-88

    McCauley, Micah J. (et al.)

  • Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein’s RNA Chaperone Activity

    Pages 89-106

    Schmidt, Tobias (et al.)

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-07-160231-7
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-1-07-160230-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
RNA Chaperones
Book Subtitle
Methods and Protocols
Editors
  • Tilman Heise
Series Title
Methods in Molecular Biology
Series Volume
2106
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160231-7
DOI
10.1007/978-1-0716-0231-7
Hardcover ISBN
978-1-07-160230-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 314
Number of Illustrations
8 b/w illustrations, 70 illustrations in colour
Topics