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Molecular Toxicology Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Phouthone Keohavong

    1. Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, USA

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  2. Kamaleshwar P. Singh

    1. Department of Environmental Toxicology, The Institute of Environmental and Human Health (TIEHH), Texas Tech University, Lubbock, USA

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  3. Weimin Gao

    1. Department of Occupational and Environmental Health Sciences, School of Public Health West Virginia University, Morgantown, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2102)

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Table of contents (31 protocols)

  1. Front Matter

    Pages i-xiv
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  2. Bioinformatics and Biostatistics

    1. Front Matter

      Pages 1-1
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    2. Bioenergetic Analyses of In Vitro and In Vivo Samples to Guide Toxicological Endpoints

      • Jonathan W. Boyd, Julia A. Penatzer, Nicole Prince, Julie V. Miller, Alice A. Han, Holly N. Currie
      Pages 3-15

    3. Next Generation Sequencing (NGS) Application in Multiparameter Gene Expression Analysis

      • Degeng Wang, Andrey L. Karamyshev
      Pages 17-34

    4. Integrated Chip-Seq and RNA-Seq Data Analysis Coupled with Bioinformatics Approaches to Investigate Regulatory Landscape of Transcription Modulators in Breast Cancer Cells

      • Jairo Ramos, Quentin Felty, Deodutta Roy
      Pages 35-59

    5. Analysis of RNA Sequencing Data Using CLC Genomics Workbench

      • Chia-Hsin Liu, Y. Peter Di
      Pages 61-113

  3. Toxicant-Induced Lung Inflammation and Lung Tumors

    1. Front Matter

      Pages 115-115
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    2. Using Bronchoalveolar Lavage to Evaluate Changes in Pulmonary Diseases

      • Marissa E. Di, Dandan Yang, Y. Peter Di
      Pages 117-128

    3. Fast and Efficient Measurement of Clinical and Biological Samples Using Immunoassay-Based Multiplexing Systems

      • Yingze Zhang, Xiaoyun Li, Y. Peter Di
      Pages 129-147

    4. Induction of Lung Tumors and Mutational Analysis in FVB/N Mice Treated with the Tobacco Carcinogen 4-(Methylnitrosamino)-1-(3-Pyridyl)-1-Butanone

      • Natalie J. Rothenberger, Laura P. Stabile
      Pages 149-160

  4. Analysis of Protein and Gene Expression and Silencing

    1. Front Matter

      Pages 161-161
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    2. Two-Dimensional Difference Gel Electrophoresis: A Gel-Based Proteomic Approach for Protein Analysis

      • Weimin Gao
      Pages 163-176

    3. Determination and Quantification of Bacterial Virulent Gene Expression Using Quantitative Real-Time PCR

      • Qiao Lin, Y. Peter Di
      Pages 177-193

    4. Using a Lentivirus-Based Inducible RNAi Vector to Silence a Gene

      • Si Chen, Dongying Li, Zhen Ren, Dianke Yu, Baitang Ning, Nan Mei et al.
      Pages 195-210

  6. Detection of Chromosomal and Genome-Wide Damage

    1. Front Matter

      Pages 235-235
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    2. Analysis of Telomere Length and Aberrations by Quantitative FISH

      • Elise Fouquerel, Patricia Opresko
      Pages 237-249

    3. Detection of Loss of Heterozygosity in Tk-Deficient Mutants from L5178Y Tk+/−-3.7.2C Mouse Lymphoma Cells

      • Xiaoqing Guo, Ying Chen, Martha M. Moore, Nan Mei
      Pages 251-270
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Keywords

About this book

This fully revised book includes new and improved protocols to analyze toxicity at the DNA, RNA, and protein levels using genomic, epigenomic, transcriptomic, and proteomic approaches. Sections of the book address key subjects such as bioinformatics and biostatistics, toxicant-induced lung inflammation and tumors, analysis of protein and gene expression and silencing, gene promoter methylation, detection of chromosomal and genome-wide damage, analysis of DNA-adducts and surrogate gene mutations, detection and characterization of cancer gene mutation, and many other areas of study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and up-to-date, Molecular Toxicology Protocols, Third Edition will prove to play an important role in generating a better understanding of the field of toxicology and its sub-disciplines.

Editors and Affiliations

  • Department of Environmental and Occupational Health, Graduate School of Public Health, University of Pittsburgh, Pittsburgh, USA

    Phouthone Keohavong

  • Department of Environmental Toxicology, The Institute of Environmental and Human Health (TIEHH), Texas Tech University, Lubbock, USA

    Kamaleshwar P. Singh

  • Department of Occupational and Environmental Health Sciences, School of Public Health West Virginia University, Morgantown, USA

    Weimin Gao

Bibliographic Information

  • Book Title: Molecular Toxicology Protocols

  • Editors: Phouthone Keohavong, Kamaleshwar P. Singh, Weimin Gao

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0223-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0222-5Published: 28 January 2020

  • Softcover ISBN: 978-1-0716-0225-6Published: 28 January 2021

  • eBook ISBN: 978-1-0716-0223-2Published: 27 January 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XIV, 576

  • Number of Illustrations: 39 b/w illustrations, 144 illustrations in colour

  • Topics: Pharmacology/Toxicology, Genetics and Genomics, Human Genetics

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