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Methods in Molecular Biology

Immobilization of Enzymes and Cells

Methods and Protocols

Editors: Guisan, J.M., Bolivar, J.M., López-Gallego, F., Rocha-Martín, J. (Eds.)

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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eBook $219.00
price for USA in USD (gross)
  • Due: January 14, 2020
  • ISBN 978-1-07-160215-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover $279.99
price for USA in USD
  • ISBN 978-1-07-160214-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme  orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.

Table of contents (32 chapters)

Table of contents (32 chapters)
  • The Science of Enzyme Immobilization

    Pages 1-26

    Guisan, Jose M. (et al.)

  • Overview of Immobilized Enzymes’ Applications in Pharmaceutical, Chemical, and Food Industry

    Pages 27-63

    Basso, Alessandra (et al.)

  • Parameters for the Evaluation of Immobilized Enzymes Under Process Conditions

    Pages 65-81

    Illanes, Andrés (et al.)

  • One-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Immobilized “Native Enzymes”

    Pages 83-92

    Guisan, Jose M. (et al.)

  • Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes

    Pages 93-107

    López-Gallego, Fernando (et al.)

Buy this book

eBook $219.00
price for USA in USD (gross)
  • Due: January 14, 2020
  • ISBN 978-1-07-160215-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
Hardcover $279.99
price for USA in USD
  • ISBN 978-1-07-160214-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Immobilization of Enzymes and Cells
Book Subtitle
Methods and Protocols
Editors
  • Jose M. Guisan
  • Juan M. Bolivar
  • Fernando López-Gallego
  • Javier Rocha-Martín
Series Title
Methods in Molecular Biology
Series Volume
2100
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-07-160215-7
DOI
10.1007/978-1-0716-0215-7
Hardcover ISBN
978-1-07-160214-0
Series ISSN
1064-3745
Edition Number
4
Number of Pages
XVI, 495
Number of Illustrations
89 b/w illustrations, 109 illustrations in colour
Topics