Immobilization of Enzymes and Cells

1. Front Matter

   Pages i-xvi

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2. The Science of Enzyme Immobilization

   • Jose M. Guisan, Fernando López-Gallego, Juan M. Bolivar, Javier Rocha-Martín, Gloria Fernandez-Lorente

   Pages 1-26

3. Overview of Immobilized Enzymes’ Applications in Pharmaceutical, Chemical, and Food Industry

   • Alessandra Basso, Simona Serban

   Pages 27-63

4. Parameters for the Evaluation of Immobilized Enzymes Under Process Conditions

   • Andrés Illanes, Lorena Wilson

   Pages 65-81

5. One-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Immobilized “Native Enzymes”

   • Jose M. Guisan, Fernando López-Gallego, Juan M. Bolivar, Javier Rocha-Martín, Gloria Fernandez-Lorente

   Pages 83-92

6. Multi-Point Covalent Immobilization of Enzymes on Glyoxyl Agarose with Minimal Physico-Chemical Modification: Stabilization of Industrial Enzymes

   • Fernando López-Gallego, Gloria Fernandez-Lorente, Javier Rocha-Martín, Juan M. Bolivar, Cesar Mateo, Jose M. Guisan

   Pages 93-107

7. Multi-Point Covalent Immobilization of Enzymes on Supports Activated with Epoxy Groups: Stabilization of Industrial Enzymes

   • Cesar Mateo, Olga Abian, Gloria Fernandez-Lorente, Benevides C. C. Pessela, Valeria Grazu, Jose M. Guisan et al.

   Pages 109-117

8. Immobilization of Enzymes on Supports Activated with Glutaraldehyde: A Very Simple Immobilization Protocol

   • Fernando López-Gallego, Jose M. Guisan, Lorena Betancor

   Pages 119-127

9. Very Strong but Reversible Immobilization of Enzymes on Supports Coated with Ionic Polymers

   • Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Claudia Ortiz, Fernando López-Gallego et al.

   Pages 129-141

10. Immobilization of Lipases by Adsorption on Hydrophobic Supports: Modulation of Enzyme Properties in Biotransformations in Anhydrous Media

    • Gloria Fernandez-Lorente, Javier Rocha-Martín, Jose M. Guisan

    Pages 143-158

11. Immobilization of Enzymes on Hetero-Functional Supports: Physical Adsorption Plus Additional Covalent Immobilization

    • Lara Trobo-Maseda, Alejandro H. Orrego, María Romero-Fernández, Jose M. Guisan, Javier Rocha-Martín

    Pages 159-174

12. Stabilization of Multimeric Enzymes via Immobilization and Further Cross-Linking with Aldehyde-Dextran

    • Cesar Mateo, Benevides C. C. Pessela, Manuel Fuentes, Rodrigo Torres, Lorena Betancor, Aurelio Hidalgo et al.

    Pages 175-187

13. Immobilization of Enzymes on Magnetic Beads Through Affinity Interactions

    • Audrey Sassolas, Akhtar Hayat, Jean-Louis Marty

    Pages 189-198

14. Immobilization of Proteins on Gold Surfaces

    • José M. Abad, Marcos Pita, Víctor M. Fernández

    Pages 199-209

15. Immobilization of Enzymes in Protein Films

    • Daniel Sánchez-deAlcázar, Mantas Liutkus, Aitziber L. Cortajarena

    Pages 211-226

16. Encapsulation of Enzymes in Porous Capsules via Particle Templating

    • Mikhail V. Zyuzin, Pedro Ramos-Cabrer, Susana Carregal-Romero

    Pages 227-241

17. Enzyme Immobilization in Wall-Coated Flow Microreactors

    • Donya Valikhani, Juan M. Bolivar, Bernd Nidetzky

    Pages 243-257

18. In Situ Immobilization of Enzymes in Biomimetic Silica

    • Erienne Jackson, Sonali Correa, Lorena Betancor

    Pages 259-270

19. Environmentally Friendly Enzyme Immobilization on MOF Materials

    • Victoria Gascón Pérez, Manuel Sánchez-Sánchez

    Pages 271-296

20. Co-Immobilization and Co-Localization of Multi-Enzyme Systems on Porous Materials

    • Alejandro H. Orrego, Fernando López-Gallego, Gloria Fernandez-Lorente, Jose M. Guisan, Javier Rocha-Martín

    Pages 297-308

This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme  orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.

• GLYOXYL SUPPORTS
• MAGNETIC NANOPARTICLES
• HYDROPHOBIC SUPPORTS
• METAL–ORGANIC FRAMEWORKS
• HETEROGENEOUS BIOCATALYSTS.

• CSIC Campus Cantoblanco, Instituto de Catalisis, Madrid, Spain

  Jose M. Guisan

• Institute of Biotech and Biochemical Engineering, Graz University of Technology, Graz, Austria

  Juan M. Bolivar

• CIC biomaGUNE, Donostia-San Sebastian, Spain

  Fernando López-Gallego

• Campus UAM-CSIC, Institute of Catatalysis, Madrid, Spain

  Javier Rocha-Martín

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