Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2098)
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Table of contents (19 protocols)
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Front Matter
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MAIT Cells in Human Tissues and Blood
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Front Matter
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Functional Analysis of MAIT Cells
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Front Matter
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Monitoring and In Vitro Amplification of MAIT Cells
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Front Matter
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About this book
This volume focuses on various methods used by researchers to study mucosal-associated invariant T (MAIT) cells in the aspects described below. The chapters are organized into Four Parts: part One looks at the function and importance of MAIT cells in health and disease. It covers methods to isolate and characterize MAIT cells from human tissues including liver, colon tumors, placenta and decidua, and endometrium. Part Two discusses MR1 independent stimulation, and looks at the activation of MAIT cells by different stimulatory agents. Part Three describes various methods to develop tools to analyze, monitor, and generate MAIT cells in vitro, including the production of MR1-tetramers and how they can be loaded with bacterial antigens and used for the detection of MAIT cells. The last part covers the use of murine models to study MAIT cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, MAIT Cells: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing field.
Chapters 9 and 17 are open access under a CC BY 4.0 license.
Editors and Affiliations
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Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden, Clinical Immunology and Transfusion Medicine, Karolinska University Hospital, Stockholm, Sweden
Helen Kaipe
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Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden
Isabelle Magalhaes
Bibliographic Information
Book Title: MAIT Cells
Book Subtitle: Methods and Protocols
Editors: Helen Kaipe, Isabelle Magalhaes
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0207-2
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0206-5Published: 03 December 2019
Softcover ISBN: 978-1-0716-0209-6Published: 03 December 2020
eBook ISBN: 978-1-0716-0207-2Published: 02 December 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 310
Number of Illustrations: 13 b/w illustrations, 41 illustrations in colour
Topics: Immunology, Cell Biology