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Cell Reprogramming for Immunotherapy

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2097)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
  2. Reprogramming T and NK Cells

    1. Front Matter

      Pages 1-1
    2. T Cell Reprogramming Against Cancer

      • Samuel G. Katz, Peter M. Rabinovich
      Pages 3-44
    3. Microfluidic Approach for Highly Efficient Viral Transduction

      • Reginald Tran, Wilbur A. Lam
      Pages 55-65
    4. Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells

      • Roddy S. O’Connor, Michael C. Milone
      Pages 83-90
    5. Engineering of Natural Killer Cells for Clinical Application

      • Noriko Shimasaki, Dario Campana
      Pages 91-105
    6. In Vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection

      • Nathan J. Schloemer, Alex M. Abel, Monica S. Thakar, Subramaniam Malarkannan
      Pages 115-123
    7. Immunomodulation of NK Cell Activity

      • Carolina I. Domaica, Jessica M. Sierra, Norberto W. Zwirner, Mercedes B. Fuertes
      Pages 125-136
  3. Reprogramming Diverse Immunocytes

    1. Front Matter

      Pages 137-137
    2. An Overview of Advances in Cell-Based Cancer Immunotherapies Based on the Multiple Immune-Cancer Cell Interactions

      • Jialing Zhang, Stephan S. Späth, Sherman M. Weissman, Samuel G. Katz
      Pages 139-171
    3. Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy

      • Douglas Hanlon, Olga Sobolev, Patrick Han, Alessandra Ventura, Aaron Vassall, Nour Kibbi et al.
      Pages 173-195
    4. Reprogramming Exosomes for Immunotherapy

      • Qinqin Cheng, Xiaojing Shi, Yong Zhang
      Pages 197-209
    5. Nanoparticles for Immune Cell Reprogramming and Reengineering of Tumor Microenvironment

      • Ketki Bhise, Samaresh Sau, Rami Alzhrani, Arun K. Iyer
      Pages 211-221
  4. Monitoring Reprogrammed Immune Cells In Vivo

    1. Front Matter

      Pages 265-265

About this book

This volume details key protocols for developing strategies in immunotherapy.  Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Pathology, Yale School of Medicine, New Haven, USA

    Samuel G. Katz, Peter M. Rabinovich

Bibliographic Information

  • Book Title: Cell Reprogramming for Immunotherapy

  • Book Subtitle: Methods and Protocols

  • Editors: Samuel G. Katz, Peter M. Rabinovich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0203-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0202-7Published: 28 November 2019

  • Softcover ISBN: 978-1-0716-0205-8Published: 28 November 2020

  • eBook ISBN: 978-1-0716-0203-4Published: 27 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 343

  • Number of Illustrations: 7 b/w illustrations, 34 illustrations in colour

  • Topics: Immunology, Cell Biology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access