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Cell Reprogramming for Immunotherapy

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Samuel G. Katz

    1. Department of Pathology, Yale School of Medicine, New Haven, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Peter M. Rabinovich

    1. Department of Pathology, Yale School of Medicine, New Haven, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2097)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Reprogramming T and NK Cells

    1. Front Matter

      Pages 1-1
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    2. T Cell Reprogramming Against Cancer

      • Samuel G. Katz, Peter M. Rabinovich
      Pages 3-44

    3. Identification of Cell Surface Targets for CAR T Cell Immunotherapy

      • Diana C. DeLucia, John K. Lee
      Pages 45-54

    4. Microfluidic Approach for Highly Efficient Viral Transduction

      • Reginald Tran, Wilbur A. Lam
      Pages 55-65

    5. Single-Cell Cytokine Analysis to Characterize CAR-T Cell Activation

      • Amanda Finck, Rong Fan
      Pages 67-81

    6. Testing the Specificity of Compounds Designed to Inhibit CPT1A in T Cells

      • Roddy S. O’Connor, Michael C. Milone
      Pages 83-90

    7. Engineering of Natural Killer Cells for Clinical Application

      • Noriko Shimasaki, Dario Campana
      Pages 91-105

    8. Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

      • Arash Nanbakhsh, Subramaniam Malarkannan
      Pages 107-113

    9. In Vivo Assessment of NK Cell-Mediated Cytotoxicity by Adoptively Transferred Splenocyte Rejection

      • Nathan J. Schloemer, Alex M. Abel, Monica S. Thakar, Subramaniam Malarkannan
      Pages 115-123

    10. Immunomodulation of NK Cell Activity

      • Carolina I. Domaica, Jessica M. Sierra, Norberto W. Zwirner, Mercedes B. Fuertes
      Pages 125-136

  3. Reprogramming Diverse Immunocytes

    1. Front Matter

      Pages 137-137
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    2. An Overview of Advances in Cell-Based Cancer Immunotherapies Based on the Multiple Immune-Cancer Cell Interactions

      • Jialing Zhang, Stephan S. Späth, Sherman M. Weissman, Samuel G. Katz
      Pages 139-171

    3. Rapid Production of Physiologic Dendritic Cells (phDC) for Immunotherapy

      • Douglas Hanlon, Olga Sobolev, Patrick Han, Alessandra Ventura, Aaron Vassall, Nour Kibbi et al.
      Pages 173-195

    4. Reprogramming Exosomes for Immunotherapy

      • Qinqin Cheng, Xiaojing Shi, Yong Zhang
      Pages 197-209

    5. Nanoparticles for Immune Cell Reprogramming and Reengineering of Tumor Microenvironment

      • Ketki Bhise, Samaresh Sau, Rami Alzhrani, Arun K. Iyer
      Pages 211-221

    6. CRISPR/Cas9 Gene Targeting in Primary Mouse Bone Marrow-Derived Macrophages

      • Will Bailis
      Pages 223-230

    7. Genome-Wide CRISPRi/a Screening in an In Vitro Coculture Assay of Human Immune Cells with Tumor Cells

      • Jialing Zhang, Stephan S. Späth, Samuel G. Katz
      Pages 231-252

    8. The Propagation and Quantification of Two Emerging Oncolytic Viruses: Vesicular Stomatitis (VSV) and Zika (ZIKV)

      • Robert E. Means, Sounak Ghosh Roy, Samuel G. Katz
      Pages 253-263

  4. Monitoring Reprogrammed Immune Cells In Vivo

    1. Front Matter

      Pages 265-265
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Keywords

About this book

This volume details key protocols for developing strategies in immunotherapy.  Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Pathology, Yale School of Medicine, New Haven, USA

    Samuel G. Katz, Peter M. Rabinovich

Bibliographic Information

  • Book Title: Cell Reprogramming for Immunotherapy

  • Book Subtitle: Methods and Protocols

  • Editors: Samuel G. Katz, Peter M. Rabinovich

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0203-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0202-7Published: 28 November 2019

  • Softcover ISBN: 978-1-0716-0205-8Published: 28 November 2020

  • eBook ISBN: 978-1-0716-0203-4Published: 27 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 343

  • Number of Illustrations: 7 b/w illustrations, 34 illustrations in colour

  • Topics: Immunology, Cell Biology

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