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Methods in Molecular Biology
cover

Animal Cell Biotechnology

Methods and Protocols

Editors: Pörtner, Ralf (Ed.)

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
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  • ISBN 978-1-0716-0191-4
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About this book

Animal Cell Biotechnology: Methods and Protocols, Fourth Edition constitutes a comprehensive manual of state-of-the-art techniques for setting up mammalian cell lines and media for development of biopharmaceuticals, and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. Special emphasis was put on model-assisted concepts. Scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in cell line and media development, optimizing process techniques and process strategies, use of model-assisted tools for process design and optimization, and in analysis. Topics include cell line and media development, techniques for process development, model-based techniques for process development, process analysis, and downstream techniques. The volume is divided into five parts that reflect the processes required for different stages of production. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Animal Cell Biotechnology: Methods and Protocols, Fourth Edition provides a compendium of techniques for scientists in industrial and research laboratories that use mammalian cells for biotechnology purposes.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • Near-Physiological Cell Cycle Synchronization with Countercurrent Centrifugal Elutriation

    Pages 3-16

    Möller, Johannes (et al.)

  • Turning Cells into Reagents: The Preparation of Assay Ready Cells for Routine Use in Bioassays

    Pages 17-25

    Wehmeier, Oliver (et al.)

  • Screening of Media Supplements for High-Performance Perfusion Cultures by Design of Experiment

    Pages 27-39

    Mayrhofer, Patrick (et al.)

  • ambr® 15 Microbioreactors for CHO Cell Cultivation

    Pages 43-67

    Warr, Steve R. C.

  • Using a Parallel Micro-Cultivation System (Micro-Matrix) as a Process Development Tool for Cell Culture Applications

    Pages 69-81

    Wiegmann, Vincent (et al.)

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-1-0716-0191-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-1-0716-0190-7
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Animal Cell Biotechnology
Book Subtitle
Methods and Protocols
Editors
  • Ralf Pörtner
Series Title
Methods in Molecular Biology
Series Volume
2095
Copyright
2020
Publisher
Springer US
Copyright Holder
Springer Science+Business Media, LLC, part of Springer Nature
eBook ISBN
978-1-0716-0191-4
DOI
10.1007/978-1-0716-0191-4
Hardcover ISBN
978-1-0716-0190-7
Series ISSN
1064-3745
Edition Number
4
Number of Pages
XIV, 399
Number of Illustrations
47 b/w illustrations, 80 illustrations in colour
Topics

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