Metabolic Flux Analysis in Eukaryotic Cells
Methods and Protocols
Editors: Nagrath, Deepak (Ed.)
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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- About this book
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This volume explores the latest metabolic flux analysis (MFA) techniques that cover the analysis of cellular, organ level, and whole-body metabolism. The chapters in this book discuss topics such as deutrium tracing; isotopologue fractions using GC-TOF; non-targeted mass isotopolome analysis; large-scale profiling of cellular metabolic activities using deep 13C labeling medium; metastases in mice; SWATH; Exo-MFA; metabolic flux from time-course metabolomics; and thermodynamic approaches in flux analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Metabolic Flux Analysis in Eukaryotic Cells: Methods and Protocols is a valuable resource for both experts in MFA techniques and researchers getting involved in the role of quantitative studies to uncover the dysregulated pathways in human diseases.
- Table of contents (17 chapters)
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Determination of Isotopologue and Tandem Mass Isotopologue Ratios Using Gas Chromatography Chemical Ionization Time of Flight Mass Spectrometry - Methodology and Uncertainty of Measurement
Pages 1-16
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Non-Targeted Mass Isotopolome Analysis Using Stable Isotope Patterns to Identify Metabolic Changes
Pages 17-32
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Liquid Chromatography Methods for Separation of Polar and Charged Intracellular Metabolites for 13C Metabolic Flux Analysis
Pages 33-50
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Deuterium Tracing to Interrogate Compartment-Specific NAD(P)H Metabolism in Cultured Mammalian Cells
Pages 51-71
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Large-Scale Profiling of Cellular Metabolic Activities Using Deep 13C Labeling Medium
Pages 73-92
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Table of contents (17 chapters)
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Bibliographic Information
- Bibliographic Information
-
- Book Title
- Metabolic Flux Analysis in Eukaryotic Cells
- Book Subtitle
- Methods and Protocols
- Editors
-
- Deepak Nagrath
- Series Title
- Methods in Molecular Biology
- Series Volume
- 2088
- Copyright
- 2020
- Publisher
- Springer US
- Copyright Holder
- Springer Science+Business Media, LLC, part of Springer Nature
- eBook ISBN
- 978-1-0716-0159-4
- DOI
- 10.1007/978-1-0716-0159-4
- Hardcover ISBN
- 978-1-0716-0158-7
- Softcover ISBN
- 978-1-0716-0161-7
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XIII, 370
- Number of Illustrations
- 23 b/w illustrations, 64 illustrations in colour
- Topics
