Methods in Molecular Biology

Neurogenetics

Methods and Protocols

Editors: Potter, Nicholas T. (Ed.)

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Hardcover $179.99
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Softcover $189.00
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  • ISBN 978-1-61737-290-2
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About this book

The rapid identification and characterization of neurologically relevant genes holds great potential for understanding not only the pathophysiologic mechanisms of neurological diseases, but also for improving their diagnosis and management. In Neurogenetics: Methods and Protocols, an international panel of recognized academic physicians, researchers, and clinical laboratory diagnosticians describe their best methods for characterizing these genes, their mutations, and their proteins. Providing detailed step-by-step instructions to ensure successful experimental results, these experts cover the key methods for mutation detection and screening, including discussions of quantitative PCR, trinucleotide repeat detection, sequence-based mutation detection, fluorescence in situ hybridization (FISH), in vitro protein expression systems, and studies of protein expression and function. Each method has been thoroughly tested and includes notes on troubleshooting and avoiding pitfalls.
Comprehensive and timely, Neurogenetics: Methods and Protocols offers academic neurologists and clinical laboratory diagnosticians an authoritative collection of readily reproducible techniques that can greatly assist with the diagnosis, management, and understanding of the pathophysiologic mechanisms of neurological diseases.

Reviews

"This book provides detailed laboratory methods in its of 32 chapters. Each chapter provides sufficient detail to allow the duplication of the laboratory method. The emphasis is on neurogenetic diseases and the methods are all molecular biological including PCR, DNA sequencing, Western Blot, and Southern Blot. Diseases covered include Huntington's disease, Charcot-Marie-Tooth, Rett's Syndrome, Leber's Optic Atrophy, spinocerebellar ataxias, Parkinson's disease, and neurological mitochondrial diseases.. This is a unique and highly useful book that details laboratory methods in more detail than any comparable book. Laboratory scientists and investigators who wish to use neurogenetic laboratory methods to study these disorders will find great value in this compendium of methodologies."-Doody's Health Sciences Book Review Journal

Table of contents (32 chapters)

  • Determination of Gene Dosage

    Prior, Thomas W.

    Pages 3-12

  • Semiquantitative PCR for the Detection of Exon Rearrangements in the Parkin Gene

    Lücking, Christoph B. (et al.)

    Pages 13-26

  • Detection of FMR1 Trinucleotide Repeat Expansion Mutations Using Southern Blot and PCR Methodologies

    Tarleton, Jack

    Pages 29-39

  • Extreme Expansion Detection in Spinocerebellar Ataxia Type 2 and Type 7

    Snow, Karen (et al.)

    Pages 41-50

  • Repeat Expansion Detection (RED) and the RED Cloning Strategy

    Yuan, Qiu-Ping (et al.)

    Pages 51-60

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-330-9
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-0-89603-990-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $189.00
price for USA in USD
  • ISBN 978-1-61737-290-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Neurogenetics
Book Subtitle
Methods and Protocols
Editors
  • Nicholas T. Potter
Series Title
Methods in Molecular Biology
Series Volume
217
Copyright
2003
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-330-9
DOI
10.1385/1592593305
Hardcover ISBN
978-0-89603-990-2
Softcover ISBN
978-1-61737-290-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 390
Topics