Methods in Molecular Biology

PCR Cloning Protocols

Editors: Chen, Bing-Yuan, Janes, Harry W. (Eds.)

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About this book

In the post-genomic era, PCR has become the method of choice not only for cloning existing genes, but also for generating a wide array of novel genes by mutagenesis and/or recombination within the genes of interest. PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long-distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination and to clone the challenging uncharacterized DNA flanking a known DNA fragment. Powerful applications of PCR in library construction and sublibrary generation and screening are also presented.
Authoritative and up-to-date, PCR Cloning Protocols, Second Edition, constitutes a gold-standard collection of the fastest, simplest, and most popular methods for isolating genes from all biological samples and creating novel genes from them by mutagenesis/recombination-essential methods for today's study of functional genomics, gene expression, protein structure-function relationships, protein engineering, and molecular evolution.

Reviews

"This hardcover book is packed full of detailed protocols and methodological articles on various aspects of PCR. The fact that the book was taken out of my hands by a colleague, to read up on touch-down PCR, as soon as I received it for review, attest to the book's usefulness. I will have to chain this book to my bookshelf, to make sure it does not disappear quickly-or I may consider buying a couple for the laboratory." -Plant Science

Review of the first edition.
This volume provides protocols useful to both the inexperienced and the experienced investigator. Most importantly it highlights the emerging role of PCR as a versatile tool for the molecular biologist eliminating many laborious and expensive techniques associated with traditional gene isolation and analysis.-Biochemical Education

Table of contents (44 chapters)

  • Polymerase Chain Reaction

    Kolmodin, Lori A. (et al.)

    Pages 3-18

  • Computer Programs for PCR Primer Design and Analysis

    Chen, Bing-Yuan (et al.)

    Pages 19-29

  • Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming

    Roux, Kenneth H.

    Pages 31-36

  • XL PCR Amplification of Long Targets from Genomic DNA

    Kolmodin, Lori A.

    Pages 37-51

  • Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments

    Aatsinki, Jyrki T.

    Pages 53-58

Buy this book

eBook $179.00
price for USA in USD (gross)
  • ISBN 978-1-59259-177-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $229.00
price for USA in USD
  • ISBN 978-0-89603-969-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $229.00
price for USA in USD
  • ISBN 978-1-61737-281-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
PCR Cloning Protocols
Editors
  • Bing-Yuan Chen
  • Harry W. Janes
Series Title
Methods in Molecular Biology
Series Volume
192
Copyright
2002
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-177-0
DOI
10.1385/1592591779
Hardcover ISBN
978-0-89603-969-8
Softcover ISBN
978-1-61737-281-0
Series ISSN
1064-3745
Edition Number
2
Number of Pages
XIV, 439
Topics