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Methods in Molecular Biology

Biopolymer Methods in Tissue Engineering

Editors: Hollander, Anthony P., Hatton, Paul V. (Eds.)

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About this book

Because the rapidly developing field of tissue engineering encompasses all the major scientific disciplines-including materials science, polymer chemistry, biology, and medicine-it has often been difficult to uncover the full range of laboratory methods required in tissue engineering. In Biopolymer Methods in Tissue Engineering, expert laboratory researchers bring together in a standard format all the diverse laboratory methods needed to perform state-of-the-art tissue engineering research. Topics range from the synthesis, processing, and characterization of specific biomaterials, through the successful use of scaffolds in the engineering of tissues, to techniques useful in evaluating the biological quality of scaffold-engineered tissues. Subjects of special interest include the incorporation of biological molecules into scaffold biomaterials and the use of a wide range of methods and techniques to generate a comprehensive description of cell-polymer construct quality. Additional techniques can be used to develop effective in vitro models for drug evaluation or to investigate cell and tissue differentiation. Each readily reproducible protocol follows a uniform format that includes essential background information, step-by-step instructions by an expert, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, Biopolymer Methods in Tissue Engineering offers both advanced and novice investigators a diverse collection of readily reproducible techniques for generating living cell-polymer constructs in their laboratories and for evaluating their biological quality.

Reviews

From the reviews:

"This small, 280-page volume is useful to people who are interested in using various laboratory techniques to conduct tissue engineering research. … Tips on troubleshooting and avoiding pitfalls which appear at the end of nearly all chapters will be welcomed by users of the book. The goal of this book is to ‘serve as a basic laboratory manual allowing tissue engineering scientists not only to access a wide range of techniques in one place, but also to have them described using a standard format’." (Annales de Chimie Science des Materiaux, Vol. 29 (4), 2004)


Table of contents (19 chapters)

Table of contents (19 chapters)
  • Processing of Resorbable Poly-α-Hydroxy Acids for Use as Tissue-Engineering Scaffolds

    Pages 1-10

    Kellomäki, Minna (et al.)

  • Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healing

    Pages 11-24

    Gorodetsky, Raphael (et al.)

  • Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering

    Pages 25-39

    Soranzo, Carlo (et al.)

  • Synthesis and Characterization of Chitosan Scaffolds for Cartilage-Tissue Engineering

    Pages 41-48

    Elder, Steven H. (et al.)

  • Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2

    Pages 49-64

    Kim, Hyun D. (et al.)

Buy this book

eBook $119.00
price for USA in USD
  • ISBN 978-1-59259-428-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
Softcover $159.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Biopolymer Methods in Tissue Engineering
Editors
  • Anthony P. Hollander
  • Paul V. Hatton
Series Title
Methods in Molecular Biology
Series Volume
238
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-428-3
DOI
10.1385/159259428X
Hardcover ISBN
978-0-89603-967-4
Softcover ISBN
978-1-61737-280-3
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XV, 257
Topics