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Methods in Molecular Biology

Cytokine Protocols

Editors: De Ley, Marc (Ed.)

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About this book

Because cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. Described in step-by-step detail to ensure successful experimental results, these protocols cover the large-scale generation and purification of plasmid DNA, the identification of DNA-protein interactions via the gel mobility shift assay, RNA-level phenomena, and the isolation and characterization of cytokines, cytokine-related proteins, and their interactions. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Each protocol includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and, where needed, a discussion of the interpretation of results.
Comprehensive and highly practical, Cytokine Protocols offers novice researchers a concise introduction to the principles of cytokine research, and experienced investigators all the advanced techniques and time-saving tips needed to ensure productive research.

Table of contents (19 chapters)

Table of contents (19 chapters)
  • Large-Scale Generation of Plasmids that Express Type I Interferon

    Pages 1-6

    Härle, Peter (et al.)

  • Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay

    Pages 7-20

    Chan, Rebecca J. (et al.)

  • Modulation of the Interferon-γ Signal by Transfection of Cells with an Antisense-RNA Expressing Vector

    Pages 21-29

    Delrue, Leen (et al.)

  • Competitive RT-PCR to Quantify Small Amounts of mRNA

    Pages 31-40

    Aust, Gabriela

  • In SituHybridization for Cytokines in Human Tissue Biopsies

    Pages 41-46

    Mathey, Emily (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-59259-667-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $149.99
price for USA in USD
  • ISBN 978-0-89603-948-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.00
price for USA in USD
  • ISBN 978-1-61737-269-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Cytokine Protocols
Editors
  • Marc De Ley
Series Title
Methods in Molecular Biology
Series Volume
249
Copyright
2004
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-667-6
DOI
10.1385/1592596673
Hardcover ISBN
978-0-89603-948-3
Softcover ISBN
978-1-61737-269-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIII, 240
Topics