Methods in Molecular Medicine

E. coli

Shiga Toxin Methods and Protocols

Editors: Philpott, Dana, Ebel, Frank (Eds.)

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About this book

Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx), also known as "hamburger E. coli" are continuing sources of significant-and sometime fatal-human and animal infections. In E. coli: Shiga Toxin Methods and Protocols, a multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of STEC and Stx pathogenesis. Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host-pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models. Each protocol has been thoroughly tested and optimized to produce robust and successful experimental results, and includes troubleshooting tips, advice on avoiding pitfalls, and alternate methods.
Comprehensive and up-to-date, E. coli: Shiga Toxin Methods and Protocols offers bacteriologists and medical microbiologists today's most powerful tools for illuminating the molecular and cellular microbiology of STEC's bacterial pathogenesis, as well as the mechanisms of its toxicity.

Reviews

"The authors should be thanked for providing this valuable resource tool that is highly recommended to microbiologists, molecular biologists and to all who are interested in bacterial toxin action. All medical libraries should house a copy of this text. The book also will serve as an excellent teaching guide for students and fellows particularly in postgraduate programs." -Folia Microbiologica

Table of contents (25 chapters)

Table of contents (25 chapters)

Buy this book

eBook $139.00
price for USA in USD
  • ISBN 978-1-59259-316-3
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-0-89603-939-1
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
Softcover $179.99
price for USA in USD
  • ISBN 978-1-61737-264-3
  • Free shipping for individuals worldwide
  • Institutional customers should get in touch with their account manager
  • Covid-19 shipping restrictions
  • Usually ready to be dispatched within 3 to 5 business days, if in stock
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Bibliographic Information

Bibliographic Information
Book Title
E. coli
Book Subtitle
Shiga Toxin Methods and Protocols
Editors
  • Dana Philpott
  • Frank Ebel
Series Title
Methods in Molecular Medicine
Series Volume
73
Copyright
2003
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-316-3
DOI
10.1385/159259316X
Hardcover ISBN
978-0-89603-939-1
Softcover ISBN
978-1-61737-264-3
Series ISSN
1543-1894
Edition Number
1
Number of Pages
XII, 352
Topics