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Methods in Molecular Biology

Developmental Biology Protocols

Volume II

Editors: Tuan, Rocky S., Lo, Cecilia W. (Eds.)

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  • ISBN 978-1-59259-065-0
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Hardcover $229.00
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Softcover $199.00
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  • ISBN 978-0-89603-575-1
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About this book

The molecular biology revolution has transformed developmental biology into one of the most exciting and fruitful fields in experimental biomedical research today. In Developmental Biology Protocols, established leaders in this field demonstrate this achievement with a comprehensive collection of cutting-edge protocols for studying and analyzing the events of embryonic development. Drawing on state-of-the-art cellular and molecular techniques, as well as new and sophisticated imaging and information technologies, this 2nd volume of three describes state-of-the-art methods for the study of organogenesis, the analysis of abnormal development and teratology, the screening and mapping of novel genes and mutations, and the application of transgenesis, including the production of transgenic animals and gene knockouts. The 1st and 3rd volumes in this seminal set complete today's widest-ranging collection of techniques designed to decipher the exact cellular, molecular, and genetic mechanisms that control the form, structure, and function of the developing embryo. Volume 1 presents readily reproducible methods for establishing and characterizing several widely used experimental model systems, for both the study of developmental patterns and morphogenesis, and the examination of embryo structure and function. In addition, there are step-by-step methods for the analysis of cell lineage, the production and use of chimeras, and the experimental molecular manipulation of embryos, including the application of viral vectors. No less innovative, volume 3 introduces powerful techniques for the manipulation of developmental gene expression and function, the analysis of gene expression, the characterization of tissue morphogenesis and development, the in vitro study of differentiation and development, and the genetic analysis of developmental models of diseases.
Highly practical and richly annotated, the three volumes of Developmental Biology Protocols describe multiple experimental systems and details techniques adopted from the broadest array of biomedical disciplines. Every researcher will not only better understand the principles, background, and rationale for how form and function are elaborated in an organism, but also gain full practical access to today's best methods for its analysis.

Reviews

"We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This
is the collection to get before your next grant application is due."-Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA

"The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done!" -Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME

"Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail. . .these protocols will be an invaluable aid to every geneticist and developmental biologist."-Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany

Table of contents (45 chapters)

  • Developmental Biology Protocols

    Tuan, Rocky S. (et al.)

    Pages 3-4

  • Drosophila as a Genetic Tool to Define Vertebrate Pathway Players

    Bonini, Nancy M.

    Pages 7-14

  • Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis

    Asashima, Makoto (et al.)

    Pages 15-26

  • Analysis of Mammary Gland Morphogenesis

    Roskelley, Calvin D. (et al.)

    Pages 27-38

  • Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro

    Yost, H. Joseph

    Pages 39-43

Buy this book

eBook $149.00
price for USA in USD (gross)
  • ISBN 978-1-59259-065-0
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $229.00
price for USA in USD
  • ISBN 978-0-89603-853-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $199.00
price for USA in USD
  • ISBN 978-0-89603-575-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Developmental Biology Protocols
Book Subtitle
Volume II
Editors
  • Rocky S. Tuan
  • Cecilia W. Lo
Series Title
Methods in Molecular Biology
Series Volume
136
Copyright
2000
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-065-0
DOI
10.1385/1592590659
Hardcover ISBN
978-0-89603-853-0
Softcover ISBN
978-0-89603-575-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XXV, 505
Topics