Developmental Biology Protocols

1. Front Matter

   Pages i-xxviii

   PDF

2. Introduction

   1. Front Matter

      Pages 1-4

      PDF

   2. Developmental Biology Protocols

      • Rocky S. Tuan, Cecilia W. Lo

      Pages 3-4

3. Organogenesis

   1. Front Matter

      Pages 5-121

      PDF

   2. Drosophila as a Genetic Tool to Define Vertebrate Pathway Players

      • Nancy M. Bonini

      Pages 7-14

   3. Bioassays for Studying the Role of the Peptide Growth Factor Activin in Early Amphibian Embryogenesis

      • Makoto Asashima, Takashi Ariizumi, Shuji Takahashi, George M. Malacinski

      Pages 15-26

   4. Analysis of Mammary Gland Morphogenesis

      • Calvin D. Roskelley, Colleen Wu, Aruna M. Somasiri

      Pages 27-38

   5. Specification of Cardiac Mesenchyme and Heart Morphogenesis In Vitro

      • H. Joseph Yost

      Pages 39-43

   6. Craniofacial Development and Patterning

      • Harold Slavkin, Glen Nuckolls, Lillian Shum

      Pages 45-54

   7. Craniofacial Skeletel Morphogenesis In Vitro

      • Roy C. Ogle

      Pages 55-59

   8. Skeletal Morphogenesis

      • Stefan Mundlos

      Pages 61-70

   9. Transplantation and Culture Techniques for the Analysis of Urodele Limb Regeneration

      • David L. Stocum

      Pages 71-78

   10. Retroviral Infection of T-Cell Precursors in Thymic Organ Culture

       • Lisa M. Spain, Lisa L. Lau, Yousuke Takahama

       Pages 79-86

   11. Assay for the Isolation of Hepatogenic Factors

       • Johannes A. A. Spijkers, Theodorus B. M. Hakvoort, Wouter H. Lamers

       Pages 87-100

   12. Skin Morphogenesis

       • Cheng-Ming Chuong

       Pages 101-106

   13. Apoptosis in Development

       • Lin Lin, Zahra F. Zakeri

       Pages 107-113

   14. Methods to Detect Patterns of Cell Death in Drosophila

       • Nancy M. Bonini

       Pages 115-121

4. Abnormal Development and Teratology

   1. Front Matter

      Pages 123-281

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   2. Mechanisms of Neurulation

      • Gary C. Schoenwolf, Jodi L. Smith

      Pages 125-134

   3. Neurulation and Neural Tube Closure Defects

      • Andrew Copp, Patricia Cogram, Angeleen Fleming, Dianne Gerrelli, Deborah Henderson, Andrew Hynes et al.

      Pages 135-160

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events—changes in form, structure, and function of the org- ism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and mole- lar biology, as well as evolutionary biology. No longer is the study of embryonic development merely “embryology.” In fact, development biology has produced - portant paradigms for both basic and clinical biomedical sciences alike. Although modern developmental biology has its roots in “experimental emb- ology” and the even more classical “chemical embryology,” the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the “cellular and molecular biology revolution.” The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

• Drosophila
• Neurulation
• Regeneration
• Vertebrate
• developmental biology
• evolution
• evolutionary biology
• genes
• physiology

"We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This
is the collection to get before your next grant application is due."-Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA

"The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done!" -Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME

"Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail. . .these protocols will be an invaluable aid to every geneticist and developmental biologist."-Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany

• Thomas Jefferson University, Philadelphia

  Rocky S. Tuan

• University of Pennsylvania, Philadelphia

  Cecilia W. Lo

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