Methods in Molecular Biology

Oxidants and Antioxidants

Ultrastructure and Molecular Biology Protocols

Editors: Armstrong, Donald (Ed.)

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About this book

It is now feasible to identify subcellular sites where oxidative stress reactions occur and to determine gene expression relevant to those complex pathways. In Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols, a panel of world authorities present cutting-edge molecular and ultrastructure techniques for investigating the pathophysiology of oxidative stress. Described in step-by-step detail, these readily reproducible methods use the latest in immunodetection, X-ray diffraction, confocal microscopy, in situ hybridization, chemiluminescence, and cytofluorescence. Additional techniques take advantage of electron paramagnetic resonance, advanced glycation endproducts, Western blotting analysis, transcription factors, p47phox translocation, and microsphere delivery systems. The collection emphasizes the visual observation of free radicals or products altered by oxidative stress, and includes numerous in vivo, ex vivo, and in vitro applications. Two companion volumes, Oxidative Stress Biomarkers and Antioxidant Protocols (2002), and Free Radical and Antioxidant Protocols (1998), focus on the related biochemical and cellular biological methods.
Authoritative and highly practical, Oxidants and Antioxidants: Ultrastructure and Molecular Biology Protocols provides a comprehensive collection of powerful ultrastructure and molecular tools for the evaluation of metabolic disorders linked to oxidative stress and the aging process.

Reviews

"...this volume is unique on its focus on protocols for identification and localization of oxidants, using molecular biology and other types of applications both in-vitro and in-vivo. This book is recommended for all biomedical research libraries and researchers at all levels who are interested in oxidative stress measurement." - E-STREAMS

Table of contents (36 chapters)

Table of contents (36 chapters)
  • Cytochemical Localization of H2O2 in Biological Tissues

    Pages 3-12

    Ellis, E. Ann (et al.)

  • Localization of Intracellular Lipid Hydroperoxides Using The Tetramethylbenzidine Reaction for Transmission Electron Microscopy

    Pages 13-17

    Ellis, E. Ann (et al.)

  • The Immunohistochemical Localization of Glutathione Peroxidase

    Pages 19-29

    Akeo, Kiyoshi (et al.)

  • 4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody

    Pages 31-34

    Majima, Hideyuki J. (et al.)

  • Immunohistochemical Detection of Protein Oxidation

    Pages 35-40

    Frank, Jürgen (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-274-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.00
price for USA in USD
  • ISBN 978-0-89603-851-6
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-61737-223-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Oxidants and Antioxidants
Book Subtitle
Ultrastructure and Molecular Biology Protocols
Editors
  • Donald Armstrong
Series Title
Methods in Molecular Biology
Series Volume
196
Copyright
2002
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-274-6
DOI
10.1385/1592592740
Hardcover ISBN
978-0-89603-851-6
Softcover ISBN
978-1-61737-223-0
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 356
Topics