Overview
- Editors:
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Tanya Parish
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St. Bartholomew’s and Royal London School of Medicine and Dentistry, London, UK
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Neil G. Stoker
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London School of Hygiene and Tropical Medicine, London, UK
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Table of contents (24 protocols)
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- Nicola Casali, Sabine Ehrt
Pages 1-17
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- Paul D. van Helden, Thomas C. Victor, Robin M. Warren, Eileen G. van Helden
Pages 19-30
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- Irene M. Monahan, Joseph A. Mangan, Philip D. Butcher
Pages 31-42
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- Stoyan S. Bardarov, Svetoslav S. Bardarov Jr., William R. Jacobs Jr.
Pages 43-57
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- Mary Jackson, Luis Reinaldo Camacho, Brigitte Gicquel, Christophe Guilhot
Pages 59-75
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- Bhavna G. Gordhan, Tanya Parish
Pages 77-92
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- Peter Sander, Burkhard Springer, Erik C. Böttger
Pages 93-104
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- Farahnaz Movahedzadeh, Jorge A. Gonzalez-Y-Merchand, Robert A. Cox
Pages 105-124
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- Rory P. Cooney, Natalie J. Garton, Michael R. Barer
Pages 125-143
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- Thomas C. Victor, Paul D. van Helden
Pages 155-164
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- Dick van Soolingen, Petra E. W. de Haas, Kristin Kremer
Pages 165-203
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- Ida Rosenkrands, Peter Andersen
Pages 205-215
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- Mamadou Daffé, Marie-Antoinette Lanée
Pages 217-227
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- Richard A. Slayden, Clifton E. Barry 3rd
Pages 229-245
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- Pauline T. Lukey, Elizabeth U. Hooker
Pages 271-280
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- Wandy Beatty, David G. Russell
Pages 281-293
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- Riccardo Manganelli, Sanjay Tyagi, Issar Smith
Pages 295-310
About this book
The aim of this book is to provide detailed protocols for studying the molecular biology of the pathogen Mycobacterium tuberculosis, and its int- actions with host cells. As established mycobacterial laboratories move - wards exploiting the genome, and laboratories with expertise in other fields apply them to mycobacteria, both traditional and novel methodologies need to be reviewed. Thus the chapters in Mycobacterium tuberculosis Protocols range from perspectives on storage of strains and safety issues to the application of the latest functional genomics technologies. The last few years have been remarkable ones for research into M. tuber- losis. The most important landmark by far has been the completion of the genome sequence of the widely studied H37Rv strain (1). We can now predict every protein and RNA molecule made by the pathogen. This information is or will soon be enriched by the addition of genome sequences of other strains from the M. tuberculosis complex: a second strain of M. tuberculosis, My- bacterium bovis, and the vaccine strain, M. bovis BCG. Valuable comparative data will also be provided by the genome sequences of Mycobacterium leprae, Mycobacterium avium, and Streptomyces coelicolor. Another recent milestone for M. tuberculosis has been the development of efficient mutagenesis me- odologies, the lack of which has been a major handicap in functional studies.
Reviews
...a very useful book for the mycobacteria research community. It is a specialist's cookbook, perfect if you want to establish a new method that is outside your scope of expertise. Individual chapters are clearly divided into introduction, materials, methods and an extensive list of notes. -- International Journal of Medical Microbiology
"For those engaged in mycobacterial research (and possibly other unrelated genera) which involves genetics, biochemistry and immunology, this textbook is essential and, moreover, I would recommend it as reading material for students undertaking molecular biology experimentation." -Today's Life Science
Editors and Affiliations
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St. Bartholomew’s and Royal London School of Medicine and Dentistry, London, UK
Tanya Parish
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London School of Hygiene and Tropical Medicine, London, UK
Neil G. Stoker
