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Methods in Molecular Medicine

Mycobacterium Tuberculosis Protocols

Editors: Parish, Tanya, Stoker, Neil G. (Eds.)

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About this book

With one-third of the world's population infected with Mycobacterium tuberculosis, over two million people a year dying from tuberculosis, and the appearance of multidrug-resistant strains, the need to understand the biology of M. tuberculosis, and so to develop new interventions, has become acute. In Mycobacterium tuberculosis Protocols, leading investigators with extensive practical knowledge and experience describe their best methods for studying this dangerous pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. These molecular techniques are suitable for research in genetics, biochemistry, microbiology, cell biology, epidemiology, and diagnostics, and are at the forefront of biological research as a whole, as well as in focused M. tuberculosis research. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start-site mapping, real-time PCR, microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities.

Reviews

...a very useful book for the mycobacteria research community. It is a specialist's cookbook, perfect if you want to establish a new method that is outside your scope of expertise. Individual chapters are clearly divided into introduction, materials, methods and an extensive list of notes. -- International Journal of Medical Microbiology

"For those engaged in mycobacterial research (and possibly other unrelated genera) which involves genetics, biochemistry and immunology, this textbook is essential and, moreover, I would recommend it as reading material for students undertaking molecular biology experimentation." -Today's Life Science

Table of contents (24 chapters)

Table of contents (24 chapters)

Buy this book

eBook $149.00
price for USA in USD
  • ISBN 978-1-59259-147-3
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
Softcover $199.99
price for USA in USD
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Bibliographic Information

Bibliographic Information
Book Title
Mycobacterium Tuberculosis Protocols
Editors
  • Tanya Parish
  • Neil G. Stoker
Series Title
Methods in Molecular Medicine
Series Volume
54
Copyright
2001
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-147-3
DOI
10.1385/1592591477
Hardcover ISBN
978-0-89603-776-2
Softcover ISBN
978-1-61737-184-4
Series ISSN
1543-1894
Edition Number
1
Number of Pages
XIV, 406
Topics

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