Overview
- Editors:
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Keith R. Mitchelson
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Australian Genome Research Facility, University of Queensland, Brisbane
Walter & Eliza Hall Institute, Parkville, Australia
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Jing Cheng
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Biochip Research and Development Center State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
Aviva Biosciences Corporation, San Diego
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Table of contents (31 protocols)
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Practical Applications for Rapid DNA Fragment Sizing and Analysis
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- H. Michael Wenz, David Dailey, Martin D. Johnson
Pages 3-17
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- Karel KlepárnÃk, Odilo M. Müeller, FrantiÅ¡ek Foret
Pages 19-39
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- Haleem J. Issaq, King C. Chan
Pages 41-45
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Practical Applications for Mutation Detection
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Front Matter
Pages 47-147
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- John M. Butler, Dennis J. Reeder
Pages 49-56
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- Konstantin Khrapko, Hilary A. Coller, Xiao-Cheng Li-Sucholeiki, Paulo C. André, William G. Thilly
Pages 57-72
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- Cecilia Gelfi, Laura Cremoresi, Maurizio Ferrari, Pier Giorgio Righetti
Pages 73-88
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- Christine A. Piggee, Barry L. Karger
Pages 89-94
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- Paola Carrera, Pier Giorgio Righetti, Cecilia Gelfi, Maurizio Ferrari
Pages 95-108
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- Kenshi Hayashi, H. Michael Wenz, Masakazu Inazuka, Tomoko Tahira, Tomonari Sasaki, Donald H. Atha
Pages 109-126
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- Takao Kasuga, Jing Cheng, Keith R. Mitchelson
Pages 135-147
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Practical Applications for Genetic Analysis
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Front Matter
Pages 149-239
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- Elaine S. Mansfield, Robert B. Wilson, Paolo Fortina
Pages 151-161
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- Dieter Schmalzing, Lance Koutny, Aram Adourian, Dan Chisholm, Paul Matsudaira, Daniel Ehrlich
Pages 163-173
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- Andrea S. Kim, Xiao-Cheng Li-Sucholeiki, William G. Thilly
Pages 175-189
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- Jerome P. Ferrance, Braden Giordano, James P. Landers
Pages 191-204
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About this book
The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures to utilize this new resource for analysis of genetic variation and for the detection of disease causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and analyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analysis are manifold. Further, the high sensitivity of detection, and the ab- ity to increase sample throughput with parallel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA-adducts and single–strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids focuses on such analytical protocols, which can be used for detection and analysis of mutations and modification, from precise DNA loci through to entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.
Reviews
"These volumes comprehensively and accurately present CE technology and techniques with an emphasis on providing an understanding of the theory and instrumentation (Vol. 1), as well as detailed examples of successful analytical protocols (both volumes)....Chapters on the use of microchip and mass spectrometric detection are also included....Although numerous experimental protocols can be found in Vol. 1, it is clear that the intent is to communicate the underlying theory and capability of CE for separations. Vol. 1 should be of interest to the novice as well as the expert. In Vol. 2, each of the 31 chapters present specific examples of the use of CE. ...Nonetheless, these two volumes of the Methods in Molecular Biology series offer a representative and needed overview of the current theory and practice of CE of nucleic acids. These texts should prove very useful to both active investigators in this area and those seeking to learn more about the use and and capability of CE." - Clinical Chemistry
Editors and Affiliations
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Australian Genome Research Facility, University of Queensland, Brisbane
Keith R. Mitchelson
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Walter & Eliza Hall Institute, Parkville, Australia
Keith R. Mitchelson
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Biochip Research and Development Center State Key Laboratory for Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China
Jing Cheng
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Aviva Biosciences Corporation, San Diego
Jing Cheng