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Methods in Molecular Medicine

Interleukin Protocols

Editors: O’Neill, Luke A. J., Bowie, Andrew (Eds.)

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eBook $139.00
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Hardcover $179.99
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Softcover $179.99
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  • ISBN 978-1-61737-162-2
  • Free shipping for individuals worldwide
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About this book

Interleukins, a family of proteins that regulate immunity and inflammation throughout the body, are also involved in autoimmune disease (rheumatoid arthritis, multiple sclerosis, and asthma); are implicated in such other conditions as cancer, myocardial infarction, and depression; and have value in the diagnosis and prognosis of many diseases. Luke O'Neill and Andrew Bowie bring together in Interleukin Protocols a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave's disease, migraine, and myocardial infarction. There are also helpful discussions of the difficulties in measuring interleukins in different biological fluids, such as sputum from asthma patients, peritoneal fluids, synovial fluid from arthritic joints, and cerbrospinal fluid from patients with meningitis. Each step-by-step method is described by a hands-on expert and includes notes on how to avoid failure.
Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.

Reviews

"this volume offers protocols for measurement of various interleukins. Most are of sufficient detail so as to serve as a laboratory guide for investigators. Investigators with a specific interest in analyzing a given interleukin will find this volume helpful. "-Doody's Health Sciences Book Review Journal

"Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics." - Journal of Investigational Allergology and Clinical immunology

"A variety of techniques is represented in 36 chapters which are written in a concise, uniform format b y authors who obviously have a lot of hands-on experience with the methods covered...If you are working in this area, put this book on a shelf in the lab and it will not be just recently started graduate students who will gracefully turn to it. - Chembiochem

"The protocols that are described in the book are user friendly, in the sense that they are not only well detailed and easy to follow but also reliable. The researchers that are naive in the field will find a lot of useful information in the book whereas the experienced ones will be able to use the book as a source of additional information on the methods already in use. A must therefore for the psychoneuroendocrinologist with a working interest in the relationships between the (neuroendocrine) brain and the immune system." -Psychoneuroendocriniology


Table of contents (36 chapters)

  • ELISAs and Interleukin Research

    Greene, Catherine

    Pages 3-15

  • Elispot Technique for Assaying Interleukins

    Hauer, Almuth Christine (et al.)

    Pages 17-28

  • Measurement of Interleukins by ELISPOT Assay with Particular Application to Dual-Color Analysis (Stardust Assay)

    Okamoto, Yoshihiro (et al.)

    Pages 29-39

  • Assaying Interleukins Intracellularly by FACS

    O’Mahony, Liam (et al.)

    Pages 41-48

  • Basic RT-PCR for Measurement of Cytokine Expression

    Bajaj-Elliott, Mona (et al.)

    Pages 49-58

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-146-6
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-0-89603-738-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-61737-162-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Interleukin Protocols
Editors
  • Luke A. J. O’Neill
  • Andrew Bowie
Series Title
Methods in Molecular Medicine
Series Volume
60
Copyright
2001
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-146-6
DOI
10.1385/1592591469
Hardcover ISBN
978-0-89603-738-0
Softcover ISBN
978-1-61737-162-2
Series ISSN
1543-1894
Edition Number
1
Number of Pages
XV, 443
Topics