Overview
- Editors:
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Min Li
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Johns Hopkins School of Medicine, Baltimore
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Table of contents (15 protocols)
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- Fumio Nakamura, Yoshio Goshima, Stephen M. Strittmatter, Susumu Kawamoto
Pages 1-18
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- Susumu Kawamoto, Satoshi Hattori, Shigeo Uchino, Masayoshi Mishina, Kenji Okuda
Pages 19-32
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- Paul L. Chazot, Miroslav Cik, F. Anne Stephenson
Pages 33-42
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- Mark A. Varney, Fen-Fen Lin, Christine Jachec, Sara P. Rao, Stephen D. Hess, Edwin C. Johnson et al.
Pages 43-59
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- Erik A. Whitehorn, William J. Dower, Min Li
Pages 61-72
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- Ronald S. Petralia, Robert J. Wenthold
Pages 73-92
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- Ian J. Reynolds, Terre A. Sharma
Pages 93-102
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- Su Zhang, Richard L. Huganir
Pages 103-111
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- Robert J. Wenthold, Jaroslav Blahos II, Kyung-Hye Huh, Ronald S. Petralia
Pages 113-119
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- Steven E. Cwirla, William J. Dower, Min Li
Pages 131-141
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- Jerry M. Wright, Robert W. Peoples
Pages 143-153
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- Hendrika M. A. VanDongen, Antonius M. J. VanDongen
Pages 155-166
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- Antonio V. Ferrer-Montiel, Mauricio Montal
Pages 167-178
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- Martin Donal Cunningham, Sara Mary Cunningham
Pages 179-188
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Back Matter
Pages 189-196
About this book
Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane signaling receptors. NMDA Receptor Protocols offers today's scientists powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.
Reviews
"...In this volume of Methods in Molecular Biology great care has been taken to present the most modern approaches to solving the mysteries of the NMDA receptor...Each chapter includes detailed protocols and highly useful 'notes' that explain possible problems, sources of technical artifacts and how to overcome them....this book is informative and practical and would serve any channel or receptor researcher well." -Molecular Biotechnology
Editors and Affiliations
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Johns Hopkins School of Medicine, Baltimore
Min Li
