Methods in Molecular Biology

NMDA Receptor Protocols

Editors: Li, Min (Ed.)

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About this book

In NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane receptors or signaling receptors.
NMDA Receptor Protocols provides today's scientists at all skill levels a diverse collection of highly reproducible molecular techniques for ion channel research and biotherapeutic development. Eminently practical and reproducible, these techniques offer powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.




Reviews

"...In this volume of Methods in Molecular Biology great care has been taken to present the most modern approaches to solving the mysteries of the NMDA receptor...Each chapter includes detailed protocols and highly useful 'notes' that explain possible problems, sources of technical artifacts and how to overcome them....this book is informative and practical and would serve any channel or receptor researcher well." -Molecular Biotechnology

Table of contents (15 chapters)

Table of contents (15 chapters)
  • Isolation of Receptor Clones by Expression Screening in Xenopus Oocytes

    Pages 1-18

    Nakamura, Fumio (et al.)

  • Expression of NMDA Receptor Channel Subunit Proteins Using Baculovirus and Herpesvirus Vectors

    Pages 19-32

    Kawamoto, Susumu (et al.)

  • Transient Expression of Functional NMDA Receptors in Mammalian Cells

    Pages 33-42

    Chazot, Paul L. (et al.)

  • Stable Expression of Human NMDA Receptors in Cultured Mammalian Cells

    Pages 43-59

    Varney, Mark A. (et al.)

  • Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells

    Pages 61-72

    Whitehorn, Erik A. (et al.)

Buy this book

eBook $119.00
price for USA in USD (gross)
  • ISBN 978-1-59259-683-6
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $159.99
price for USA in USD
  • ISBN 978-0-89603-713-7
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $159.99
price for USA in USD
  • ISBN 978-1-61737-146-2
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
NMDA Receptor Protocols
Editors
  • Min Li
Series Title
Methods in Molecular Biology
Series Volume
128
Copyright
1999
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-683-6
DOI
10.1385/1592596835
Hardcover ISBN
978-0-89603-713-7
Softcover ISBN
978-1-61737-146-2
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 196
Topics