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Epstein-Barr Virus Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 174)

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Table of contents (43 protocols)

  1. Front Matter

    Pages i-xiv
  2. Viral Detection

    1. Front Matter

      Pages 77-77
    2. In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products

      • Gerald Niedobitek, Hermann Herbst
      Pages 79-91
    3. Detection of EBV Infection at the Single-Cell Level

      • Gregory J. Babcock, Emily M. Miyashita-Lin, David A. Thorley-Lawson
      Pages 103-110
    4. Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo

      • Lisa L. Decker, Gregory J. Babcock, David A. Thorley-Lawson
      Pages 111-116
  3. Culture Methods

    1. Front Matter

      Pages 117-117
    2. Virus Isolation

      • Lindsey M. Hutt-Fletcher, Susan M. Turk
      Pages 119-123
    3. Generation of Lymphoblastoid Cell Lines (LCLs)

      • Teresan Frisan, Victor Levitsky, Maria Masucci
      Pages 125-127
    4. Transient Gene Expression and MACS Enrichment

      • Dieter Kube, Martina Vockerodt
      Pages 155-164

About this book

The discovery of Epstein-Barr virus (EBV) by Epstein, Achong, and Barr, reported in 1964 (Lancet 1:702–703), was stimulated by Denis Burkitt’s rec- nition of a novel African childhood lymphoma and his postulation that an infectious agent was involved in the tumor’s etiology (Nature194:232–234, 1962). Since then, molecular and cellular biological and computational technologies have progressed by leaps and bounds. The advent of recombinant DNA technology opened the possibilities of genetic research more than most would have realized. Not only have the molecular tools permitted the analyses of viral mechanisms, but, importantly, they have formed the basis for discerning viral presence and, subsequently, viral involvement in an increasing number of diseases. Though in every field of science the search for further knowledge is likely to be a limitless phenomenon, the distinct goal in EBV research, namely, to gain sufficient insight into the viral–host interaction to be able to intercept the pathogenic process, is beginning to be realized. Epstein-Barr virus research has effectively entered the postgenomic era that began with the sequencing of the first strains, cloned in the mid to late 1980s.

Reviews

"Timely and highly practical, Epstein-Barr Virus Protocols provides powerful tools for elucidating life cycle of EBV and its host interactions, work that promises the emergence of major new treatments and cures of EVB-associated diseases, including several forms of human cancer." - Anticancer Research

"Overall it is an excellent resource aimed at postgraduate students and young researchers. Protocols are extensively referenced to the primary literature at the end of each chapter." -Today's Life Science

"This book provides a well-written and concise account of many of the protocols currently in use for the study of the Epstein-Barr virus and its related diseases. It is comprehensive..." "Both volumes are laid out attractively...and contain much valuable information on the pharmacological aspects of neurotoxicology, as well as state-of-the-art methodology." -Microbiology Today

"Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize, and treatment design." -Tumori

"This book is highly recommended for collections serving researchers in the area of molecular biology." -E-Streams

Editors and Affiliations

  • I.B.L.S. Division of Molecular Genetics, University of Glasgow, UK

    Joanna B. Wilson

  • I.B.L.S. Division of Biochemistry and Molecular Biology, University of Glasgow, UK

    Gerhard H. W. May

Bibliographic Information

  • Book Title: Epstein-Barr Virus Protocols

  • Editors: Joanna B. Wilson, Gerhard H. W. May

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592279

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2001

  • Hardcover ISBN: 978-0-89603-690-1Published: 23 March 2001

  • Softcover ISBN: 978-1-61737-137-0Published: 10 November 2010

  • eBook ISBN: 978-1-59259-227-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 438

  • Topics: Immunology

Buy it now

Buying options

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access