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Methods in Molecular Medicine

Molecular Pathology Protocols

Editors: Killeen, Anthony A. (Ed.)

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eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-59259-081-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-0-89603-681-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $229.00
price for USA in USD
  • ISBN 978-1-61737-131-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

Because molecular pathology has only recently emerged from the laboratory, many molecular pathology protocols are still to be found only in the primary literature. In Molecular Pathology Protocols, Anthony Killeen has assembled a collection of readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. Each method is described in step-by-step detail by a leading molecular pathologist or laboratory scientist who has developed it or used it extensively. These clinical laboratory techniques can be used for the diagnosis or monitoring of cancer, hematological malignancies, infectious diseases, and selected genetic disorders. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays. Time-tested practices and numerous tips on how to avoid pitfalls ensure robust and successful results.
Comprehensive and path-breaking, Molecular Pathology Protocols will enable clinical laboratories to introduce new molecular pathology tests and lay the groundwork for a much-needed standardization in this rapidly developing field.

Reviews

"It contains cutting-edge molecular diagnostic techniques with detailed step-by-step instructions and tips to obtain successful results....the purpose here is to offer readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. It is a worthy objective and the editor meets it very well....Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology....Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays....The significant diagnostic utility of molecular protocols in cancer, hermatological malignancies, and virus related diseases is covered well....It is a good protocol resource book in molecular pathology."-Doody's Health Sciences Book Review Journal

"Molecular Pathology Protocols fills a unique niche for general molecular pathology laboratories that may be called upon to perform a wide variety of tests for infectious and noninfectious diseases. It has 35 chapters that provide extensive detail on laboratory protocols for a variety of important conditions, from techniques for preparation of nucleic acid templates and mutation detection for genetic and neoplastic evaluations, to hematological applications, to a limited number of detailed protocols for infectious disease diagnosis and monitoring...The major strength of the protocols discussed in this book lies in their enormous detail and the practical remarks of the authors, which will assist in establishing the assays in a new laboratory. Another great value in some chapters is the emphasis on the assessment of quality control that will meet or exceed all the requirements of agencies that license and regulate clinical laboratories." - Clinical Infectious Diseases

"A must for genetic laboratories and all those using the new molecular biological methods." - Journal of Endocrine Genetics

"Each protocol in this book includes sections of introduction, materials, methods, and notes. Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology. Most protocols use polymerase chain reaction (PCR) based techniques. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in sity hybridization assays. This book has good references in both background of diseases and techniques. Most charts and labels have high quality;...The significant diagnostic utility of molecular protocols in cancer, hematological malignancies, and virus related diseases is covered well....This book is well written and the molecular protocols introduced can be easily followed practically." - E-Streams

Table of contents (35 chapters)

  • DNA Extraction from Paraffin-Embedded Tissues

    Fan, Hongxin (et al.)

    Pages 1-4

  • DNA Extraction from Fresh or Frozen Tissues

    Fan, Hongxin (et al.)

    Pages 5-10

  • RNA Extraction from Fresh or Frozen Tissues

    Fan, Hongxin (et al.)

    Pages 11-14

  • Single-Strand Conformation Polymorphism Analysis of Mutations in Exons 4-8 of the TP53 Gene

    Vähäkangas, Kirsi H. (et al.)

    Pages 15-27

  • Cleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation Detection

    Heisler, Laura (et al.)

    Pages 29-42

Buy this book

eBook $169.00
price for USA in USD (gross)
  • ISBN 978-1-59259-081-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-0-89603-681-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $229.00
price for USA in USD
  • ISBN 978-1-61737-131-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Molecular Pathology Protocols
Editors
  • Anthony A. Killeen
Series Title
Methods in Molecular Medicine
Series Volume
49
Copyright
2001
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-081-0
DOI
10.1385/1592590810
Hardcover ISBN
978-0-89603-681-9
Softcover ISBN
978-1-61737-131-8
Series ISSN
1543-1894
Edition Number
1
Number of Pages
XIV, 491
Topics