Celiac Disease

1. Front Matter

   Pages i-xi

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2. Celiac Disease

   • Debbie Williamson, Michael N. Marsh

   Pages 1-9

3. Genotyping Methodologies

   • Stephen Bevan, Richard S. Houlston

   Pages 11-20

4. From Linkage to Genes

   • Stephen Bevan, Richard S. Houlston

   Pages 21-31

5. Linkage and the Transmission Disequilibrium Test in Complex Traits

   • Stephen Bevan, Richard S. Houlston

   Pages 33-54

6. Extraction, Separation, and Purification of Wheat Gluten Proteins and Related Proteins of Barley, Rye, and Oats

   • Arthur S. Tatham, Simon M. Gilbert, Roger J. Fido, Peter R. Shewry

   Pages 55-73

7. Structure Elucidation of Gluten-Derived Peptides by Tandem Mass Spectrometry

   • Peter A. van Veelen, Jan W. Drijfhout, Frits Koning

   Pages 75-88

8. Synthetic Peptide Libraries for T-Cell Epitope Identification

   • Hoebert S. Hiemstra, Jan W. Drijfhout, Frits Koning

   Pages 89-96

9. Characterization of HLA-DQ-Specific Peptide-Binding Motifs

   • Yvonne van de Wal, Reinout Amons, Frits Koning

   Pages 97-103

10. Studies of Gliadin-Specific T-Cells in Celiac Disease

    • øyvind Molberg, Stephen N. McAdam, Knut E. A. Lundin, Ludvig M. Sollid

    Pages 105-124

11. Morphometric Analysis of Intestinal Mucosa

    • Michael N. Marsh, Peter T. Crowe, Kieran J. Moriarty, Arzu Ensari

    Pages 125-145

12. Morphometry of Rectal Mucosa

    • Arzu Ensari, Michael N. Marsh, Peter T. Crowe, Kieran Moriarty

    Pages 147-162

13. Organ Culture of Rectal Mucosa

    • Giuseppe Mazzarella, Francesco Paparo, Maria Maglio, Riccardo Troncone

    Pages 163-173

14. In Situ Hybridization

    • Conrad G. Beckett, Paul J. Ciclitira

    Pages 175-183

15. Measurements of Cytokine mRNA Expression by Quantitative Polymerase Chain Reaction in Studies of Celiac Disease

    • Ellen M. Nilsen, Finn-Eirik Johansen, Per Brandtzaeg

    Pages 185-202

16. Immunohistochemistry in Research and Diagnosis of Celiac Disease

    • Per Brandtzaeg, Frode L. Jahnsen

    Pages 203-239

17. Immunoassay for Detection of IgA Antitissue Transglutaminase in Patients with Celiac Disease

    • Walburga Dieterich, Ernst Otto Riecken, Detlef Schuppan

    Pages 241-245

18. Antiendomysial and Antigliadin Antibody Tests and Diagnosis of Celiac Disease

    • David J. Unsworth

    Pages 247-255

19. Whole Gut Lavage Fluid Analysis

    • Subrata Ghosh, Anna Dahele, Hazel E. Drummond, Syed S. Hoque, Kenneth Humphreys, Ian D. R. Arnott

    Pages 257-277

20. Back Matter

    Pages 279-288

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Research into gluten sensitivity has never been more popular nor more exciting. Thus a call for a new book, Celiac Disease: Methods and Protocols, devoted entirely to techniques and technology seemed a most appropriate undertaking. I am therefore grateful to Professor J. M. Walker for inviting me to complete this task for Humana Press. To do this would have been imp- sible without the contributions of friends and colleagues from around the world who have devoted so much interest to the project. It has also been necessary for them to master the unique chapter-writing skills required of every ma- script published in this series of laboratory monographs. With regard to gluten sensitivity we are in a period of great change, occasioned by the introduction of reproducible methods for cloning lymp- cytes, the application of physical methods to identify gluten sequences as T-cell antigens, the study of peptide responses in vitro and in vivo by either jejunal or rectal challenge, elucidating the locations of other genes concerned in pathogenesis, or the use of elegant immunohistocytochemical and mRNA probing techniques for analyzing the finer points of the mucosal inflam- tory response to gluten.

"Of interest to gastroenterologists, pathologists and those working in research on celiac disease, a disease linked by a so far unknown mechanism to type 1 diabetes." -Journal of Pediatric Endocrinology & Metabolism

• Department of Medicine, University of Manchester, Salford, UK

  Michael N. Marsh

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