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- About this book
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In is now understood that the response of mammalian cells to a wide variety of potentially toxic agents may be intimately linked with many human diseases, including rheumatoid arthritis, ischemia, fever, infection, and cancer. In Stress Response: Methods and Protocols, Stephen Keyse has assembled a diverse collection of readily reproducible methods devoted to the study of these varied and powerful responses. Written by leading researchers expert in the techniques they describe, these detailed methods cover the detection and assay of stress-induced damage, the activation of a wide range of signal transduction pathways by cellular stress, stress-induced gene expression, and stress protein function. To ensure experimental success, step-by-step guidance is provided for each method, along with details of reagents, equipment, and other requirements. The methods include both well-established techniques and new technologies at the leading edge of research.
Wide ranging and highly practical, Stress Response: Methods and Protocols provides a gold-standard bench manual for today's basic and clinical scientists working to understand how cells and tissues respond during physiological stress and in human disease - Reviews
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"This handbook will be very helpful in this respect for everybody interested in studies on the effects of stress at the cellular level using biochemical and molecular biology methods." -Cell Biology International
- Table of contents (28 chapters)
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Identifying and Counting Protein Modifications Triggered by Nitrosative Stress
Pages 3-14
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Determination of Carbonyl Groups in Oxidized Proteins
Pages 15-24
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Quantitation of 4-Hydroxynonenal Protein Adducts
Pages 25-34
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Detection of Oxidative Stress in Lymphocytes Using Dichlorodihydrofluorescein Diacetate
Pages 35-47
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The Measurement of Protein Degradation in Response to Oxidative Stress
Pages 49-60
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Table of contents (28 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Stress Response
- Book Subtitle
- Methods and Protocols
- Editors
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- Stephen M. Keyse
- Series Title
- Methods in Molecular Biology
- Series Volume
- 99
- Copyright
- 2000
- Publisher
- Humana Press
- Copyright Holder
- Humana Press
- eBook ISBN
- 978-1-59259-054-4
- DOI
- 10.1385/1592590543
- Hardcover ISBN
- 978-0-89603-611-6
- Softcover ISBN
- 978-1-61737-097-7
- Series ISSN
- 1064-3745
- Edition Number
- 1
- Number of Pages
- XVI, 488
- Topics
