Methods in Molecular Biology

Lipase and Phospholipase Protocols

Editors: Doolittle, Mark (Ed.)

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About this book

Lipase and Phospholipase Protocols is an important new compilation of cutting-edge techniques and strategies for the characterization, purification, and expression of mammalian lipases and phospholipases. The methods include the preparation of a variety of radiolabeled and fluorescent substrates for the determination of lipolytic activity, proven strategies for the expression and purification of recombinant lipases and phospholipases, and the characterization of lipases by such techniques as immunodetection, subunit size determination, lipase kinetics, and ligand interactions. In addition, there are methods for the molecular and genetic analysis of the lipases, the quantification of mRNA levels, and in vitro-coupled transcription/translation. The protocols cover more than a dozen specific enzymes, and many can be applied directly to all lipases and phospholipases.

Lipase and Phospholipase Protocols constitutes a highly useful single-volume handbook of basic procedures and strategies to investigate this unique class of enzymes. Its step-by-step format works perfectly at the laboratory bench or by the computer, providing practical information and examples that will lead to successful design and execution of specific techniques. It not only furnishes a technical framework for the investigation of known lipases and phospholipases, but provides well-established methods readily daptable to the discovery of new lipase enzymes.



Reviews

"...The book can be recommended to those scientists who are deeply engaged in lipid chemistry and analysis as well as enzymology. It provides a detailed listing of literature and other sources. Thus it can be successfully used as an introduction to new research fields and experimental techniques..."-Nahrung

"In view of the rapid increase in the number of new lipases and phospholipases that have been recently identified, the focus of this book is timely...In this volume, modern, up-to-date protocols with sufficient detail for experienced or peripheral investigators in this area are provided, and should prove to be a valuable resource."-The Quarterly Review of Biology

Table of contents (30 chapters)

Table of contents (30 chapters)
  • Phospholipase A2 and Phosphatidylinositol-Specific Phospholipase C Assays by HPLC and TLC with Fluorescent Substrate

    Pages 1-6

    Hendrickson, H. Stewart

  • Fluorometric Phospholipase Assays Based on Polymerized Liposome Substrates

    Pages 7-17

    Cho, Wonhwa (et al.)

  • Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate

    Pages 19-29

    Hermetter, Albin

  • Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2

    Pages 31-38

    Cho, Wonhwa (et al.)

  • Determination of Plasmalogen-Selective Phospholipase A2 Activity by Radiochemical and Fluorometric Assay Procedures

    Pages 39-47

    Farooqui, Akhlaq A. (et al.)

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-581-5
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-0-89603-546-1
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-61737-069-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Lipase and Phospholipase Protocols
Editors
  • Mark Doolittle
Series Title
Methods in Molecular Biology
Series Volume
109
Copyright
1999
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-581-5
DOI
10.1385/1592595812
Hardcover ISBN
978-0-89603-546-1
Softcover ISBN
978-1-61737-069-4
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVII, 362
Topics