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Methods in Molecular Biology

Protein Lipidation Protocols

Editors: Gelb, Michael H. (Ed.)

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About this book

In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a "hands-on" format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered.
Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.





Table of contents (17 chapters)

Table of contents (17 chapters)
  • In Vitro Analysis of GPI Biosynthesis in Mammalian Cells

    Pages 1-12

    Stevens, Victoria L.

  • Selection of Mammalian Cell Mutants in GPI Biosynthesis

    Pages 13-22

    Stevens, Victoria L.

  • Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins

    Pages 23-36

    Mayor, Satyajit

  • Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins

    Pages 37-49

    Kenworthy, Anne K. (et al.)

  • Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Kinases, eNOS, and G-Protein α-, β-, and γ-Subunits

    Pages 51-60

    Lisanti, Michael P. (et al.)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-59259-264-7
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $199.99
price for USA in USD
  • ISBN 978-0-89603-534-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4899-4330-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Protein Lipidation Protocols
Editors
  • Michael H. Gelb
Series Title
Methods in Molecular Biology
Series Volume
116
Copyright
1999
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-264-7
DOI
10.1385/1592592643
Hardcover ISBN
978-0-89603-534-8
Softcover ISBN
978-1-4899-4330-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
X, 240
Number of Illustrations
35 b/w illustrations
Topics