Skip to main content
SpringerLink
Log in

Protein Lipidation Protocols

  • Book
  • © 1999

Overview

Editors:
  1. Michael H. Gelb

    1. University of Washington, Seattle

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 116)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. In Vitro Analysis of GPI Biosynthesis in Mammalian Cells

    • Victoria L. Stevens
    Pages 1-12

  3. Selection of Mammalian Cell Mutants in GPI Biosynthesis

    • Victoria L. Stevens
    Pages 13-22

  4. Analysis of the Cell-Surface Distribution of GPI-Anchored Proteins

    • Satyajit Mayor
    Pages 23-36

  5. Imaging Fluorescence Resonance Energy Transfer as Probe of Membrane Organization and Molecular Associations of GPI-Anchored Proteins

    • Anne K. Kenworthy, Michael Edidin
    Pages 37-49

  6. Purification of Caveolae-Derived Membrane Microdomains Containing Lipid-Anchored Signaling Molecules, Such as GPI-Anchored Proteins, H-Ras, Src-Family Tyrosine Kinases, eNOS, and G-Protein α-, β-, and γ-Subunits

    • Michael P. Lisanti, Massimo Sargiacomo, Philipp E. Scherer
    Pages 51-60

  7. Analysis of Lipids in Caveolae

    • Jun Liu, Jan E. Schnitzer
    Pages 61-72

  8. Analysis of the Carbohydrate Components of Glycosylphosphatidylinositol Structures Using Fluorescent Labeling

    • Nicole Zitzmann, Michael A. J. Ferguson
    Pages 73-89

  9. Analysis of the Lipid Moiety of GPI Anchor in the Yeast Saccharomyces cerevisiae

    • Fulvio Reggiori, Elisabeth Canivenc-Gansel, Andreas Conzelmann
    Pages 91-105

  10. Rapid Identification of Cysteine-Linked Isoprenyl Groups by Metabolic Labeling with [3H]Farnesol and [3H]Geranylgeraniol

    • Douglas A. Andres, Dean C. Crick, Brian S. Finlin, Charles J. Waechter
    Pages 107-123

  11. Incorporation of Radiolabeled Prenyl Alcohols and Their Analogs into Mammalian Cell Proteins

    • Alberto Corsini, Christopher C. Farnsworth, Paul McGeady, Michael H. Gelb, John A. Glomset
    Pages 125-144

  12. Reconstitution of Yeast Farnesyltransferase from Individually Purified Subunits

    • Jun Urano, Fuyuhiko Tamanoi
    Pages 145-159

  13. Probing the Role of H-Ras Lipidation for Signaling Functions in Xenopus laevis Oocytes

    • Thomas Dudler, Michael H. Gelb
    Pages 161-176

  14. Fluorescence Measurement of Lipid-Binding Affinity and Interbilayer Transfer of Bimane-Labeled Lipidated Peptides

    • John R. Silvius
    Pages 177-186

  15. Determination of the Kinetics of Intervesicle Transfer and Transbilayer Diffusion of Bimane-Labeled Lipidated Peptides

    • John R. Silvius
    Pages 187-191

  16. Preparation and Assay of Myristoyl-CoA:Protein N-Myristoyltransferase

    • Rajala V. S. Raju, Rajendra K. Sharma
    Pages 193-211

  17. Metabolic Labeling of Protein-Derived Lipid Thioesters in Palmitoyl-Protein Thioesterase-Deficient Cells

    • Sandra L. Hofmann, Jui-Yun Lu
    Pages 213-219

  18. Fatty Acid Analysis of Protein-Derived Lipid Thioesters Isolated from Palmitoyl-Protein Thioesterase-Deficient Cells

    • Sandra L. Hofmann, Linda A. Verkruyse
    Pages 221-228

  19. Back Matter

    Pages 229-240
    Download chapter PDF
Back to top

About this book

In Protein Lipidation Protocols, Michael Gelb brings together a collection of readily reproducible techniques for studying protein lipidation, the covalent attachment of lipids to proteins. These cutting-edge methods-many never published before in a "hands-on" format-deal with glycosyl phosphatidylinositol (GPI)-containing compounds, protein fatty acylation, and protein prenylation. Included are novel techniques for determining the chemical structure of GPI-anchors, for radiolabeling the prenyl groups of protein in eukaryotic cells, a tool for developing inhibitors of the protein farnesyltransferase, and for an exciting lysosomal enzyme that cleaves fatty acyl groups from proteins, the first fatty acylase discovered.
Protein Lipidation Protocols offers biochemists, cell and molecular biologists, medicinal chemists, and pharmaceutical researchers state-of-the-art tools for understanding the complex biochemistry of protein lipidation, as well as catalyzing the development of many important new biopharmaceuticals, including anticancer drugs.





Editors and Affiliations

  • University of Washington, Seattle

    Michael H. Gelb

Bibliographic Information

  • Book Title: Protein Lipidation Protocols

  • Editors: Michael H. Gelb

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592592643

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 1999

  • Hardcover ISBN: 978-0-89603-534-8Published: 20 May 1999

  • Softcover ISBN: 978-1-4899-4330-9Published: 14 August 2013

  • eBook ISBN: 978-1-59259-264-7Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 240

  • Number of Illustrations: 35 b/w illustrations

  • Topics: Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation