Methods in Molecular Biology

Retinoid Protocols

Editors: Redfern, Christopher (Ed.)

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About this book

Christopher Redfern brings together in Retinoid Protocols a comprehensive collection of key biochemical and molecular methods for retinoid research. These easily reproducible techniques range from methods of handling and analyzing retinoids to advanced protocols for gene targeting, from binding protein function in transgenic animals to the study of nuclear retinoid receptors. In addition, the book makes accessible to all retinoid researchers such advanced techniques as cloning via RT-PCR, fluorimetry methods, recombinant protein purification and characterization, the use of antisense oligonucleotides to study the role of CRABPs, immunological and in situ hybridization methods, photoaffinity labeling, gel-shift analysis, and differential display.

Many of the chapters in Retinoid Protocols represent landmarks in retinoid methodology because they give step-by-step instructions for techniques central to understanding cellular control by retinoids. These indispensable techniques will be fundamental for all those working with retinoids, and so will play a key role in the development of new drugs and therapies for treating human disease.

Table of contents (28 chapters)

  • Properties of Retinoids

    Barua, Arun B. (et al.)

    Pages 3-28

  • Quantitative Analyses of Naturally Occurring Retinoids

    Napoli, Joseph L. (et al.)

    Pages 29-40

  • Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell Lines

    Wagner, Michael

    Pages 41-53

  • Immunohistochemistry for CRBPs and CRABPs

    Eriksson, Ulf (et al.)

    Pages 57-66

  • Whole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acid

    Lyn, Sally D.

    Pages 67-79

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-573-0
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.00
price for USA in USD
  • ISBN 978-0-89603-438-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.00
price for USA in USD
  • ISBN 978-1-4899-4244-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Retinoid Protocols
Editors
  • Christopher Redfern
Series Title
Methods in Molecular Biology
Series Volume
89
Copyright
1998
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-573-0
DOI
10.1385/0896034380
Hardcover ISBN
978-0-89603-438-9
Softcover ISBN
978-1-4899-4244-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XVI, 434
Topics