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Methods in Molecular Biology

Lipoprotein Protocols

Editors: Ordovas, Jose M. (Ed.)

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About this book

In Lipoprotein Protocols, Jose Ordovas compiles a cutting-edge collection of molecular protein techniques for studying advanced aspects of lipoprotein structure and metabolism. These step-by-step methods allow both the beginning and the advanced researcher to carry out not only fundamental, but also the most advanced genetic screening techniques, including RT-PCR and the preparation of relevant models of transgenic and knockout mice. In addition, novel techniques are presented to characterize plasma lipoproteins, their subfractions, and their modifications, and to determine the activity of relevant enzymes and carrier proteins. Each method is described in easily reproducible detail by hands-on experts who have perfected it.

Lipoprotein Protocols offers many powerful tools for unraveling the complexity of lipoprotein metabolism and determining the individual risk of coronary heart disease. For
all those basic and clinical investigators seeking to advance the frontier of lipoprotein research and develop novel genetic therapies for the treatment of cardiovascular disease, this eminently useful book constitutes the new standard reference and guide.





Reviews

"This text makes a good selection with admirable breadth and a high general quality of method description."-Clin Chem Lab Med

"The book presents the state-of-art of molecular biology and protein techniques specific for lipoprotein research...The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists..."-Nahrung

"Each chapter contains, besides a description of the principle of the methods, highly instructive protocols and lists of the technical requirements and reagents. Notes with additional information, e.g. special experimental conditions or safety and technical advises as well as numerous references are given. The book may be especially recommended for students who start working on lipoproteins, but may also be generally helpful for molecular biologists."-Nahrung

Table of contents (18 chapters)

Table of contents (18 chapters)
  • PCR Methodology Applied to Genetic Studies of Lipoprotein Metabolism and Atherosclerosis

    Pages 1-34

    Cole, Shelley A. (et al.)

  • Separation of Small-Size DNA Fragments Using Agarose Gel Electrophoresis

    Pages 35-42

    Ordovas, Jose M.

  • Quantification of mRNA by Polymerase Chain Reaction (PCR) Using an Internal Standard and a Nonradioactive Detection Method

    Pages 43-61

    Karge, William H., III (et al.)

  • Generation of Transgenic Mice

    Pages 63-78

    Hofker, Marten H. (et al.)

  • Preparation of Knockout Mice

    Pages 79-92

    Osada, Jesús (et al.)

Buy this book

eBook $129.00
price for USA in USD (gross)
  • ISBN 978-1-59259-582-2
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $169.99
price for USA in USD
  • ISBN 978-0-89603-420-4
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
Softcover $169.99
price for USA in USD
  • ISBN 978-1-61737-031-1
  • Free shipping for individuals worldwide
  • Immediate ebook access, if available*, with your print order
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Lipoprotein Protocols
Editors
  • Jose M. Ordovas
Series Title
Methods in Molecular Biology
Series Volume
110
Copyright
1998
Publisher
Humana Press
Copyright Holder
Humana Press
eBook ISBN
978-1-59259-582-2
DOI
10.1385/1592595820
Hardcover ISBN
978-0-89603-420-4
Softcover ISBN
978-1-61737-031-1
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 284
Topics

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