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Biological Methods

Enzymatic Analysis

A Practical Guide

Authors: Passonneau, Janet V., Lowry, Oliver H.

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About this book

Enzymatic Analysis: A Practical Guide is a multipurpose manual of laboratory methods. It offers a systematic scheme for the analysis of biological materials from the level of the whole organ down to the single cell and beyond. It is intended as a guide to the development of new methods, to the refinement of old ones, and to the adaptation in general of methods to almost any scale of sensitivity. As some may realize, the book is a sequel to A Flexible System of Enzymatic Analysis, originally published in 1972. The major changes, other than an appropriate interchange of authors, consist of a wholly new chapter of methods and protocols for measuring enzymes, the addition of 13 new entries in the metabolite chapter, and a much superior chapter on enzymatic cycling. With considerable nostalgia, we have switched from DPN and TPN to NAD and NADP nomenclature, which no doubt will make Otto Warburg turn over in his grave. The incentives for the methodology in this book came from the rigorous demands of quantitative histochemistry and cytochemistry. These demands are specificity, simplicity, flexibility, and, of course, sensitivity—all likewise desirable attributes of methods for other purposes. The specificity is provided by the use of enzyme methods. Simplicity is achieved by leading all reactions to a final pyridine nucleotide step.

Reviews

...the authors are well recognized for their contributions to histochemistry...For individuals interested in...performing quantitative histochemical methods, this book would be a worthwhile investment.-Modern Pathology

Table of contents (12 chapters)

Table of contents (12 chapters)

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-60327-407-4
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-0-89603-238-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4899-4014-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Enzymatic Analysis
Book Subtitle
A Practical Guide
Authors
Series Title
Biological Methods
Copyright
1993
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media LLC
eBook ISBN
978-1-60327-407-4
DOI
10.1007/978-1-60327-407-4
Hardcover ISBN
978-0-89603-238-5
Softcover ISBN
978-1-4899-4014-8
Edition Number
1
Number of Pages
X, 403
Topics