Methods in Molecular Biology

Microscopy, Optical Spectroscopy, and Macroscopic Techniques

Authors: Jones, Christopher, Mulloy, Barbara, Thomas, Adrian H.

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About this book

This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec­ troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand­ ing for the biochemist or biologist who needs to collaborate with spe­ cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol­ vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc­ ture using circular dichroism and Fourier-transform infrared spec­ troscopy.

Reviews

...a quite fascinating book. It describes a range of techniques with which I am familiar in the literature, but have never actually used and explains the practicalities behind the instrumentation and its use...very readable and informative.-Dave Roberts, Natural History Museum-Society for General Microbiology Quarterly

Table of contents (16 chapters)

Table of contents (16 chapters)
  • Electron Microscopy of Protein-Nucleic Acid Complexes

    Pages 1-12

    Gray, Carla W.

  • Visualization of Unshadowed DNA by Electron Microscopy

    Pages 13-23

    Gray, Carla W.

  • Biological Applications of Scanning Tunneling Microscopy

    Pages 25-37

    Williams, Phillip M. (et al.)

  • High-Flux X-Ray and Neutron Solution Scattering

    Pages 39-60

    Perkins, Stephen J.

  • Determination of Macromolecular Homogeneity, Shape, and Interactions Using Sedimentation Velocity Analytical Ultracentrifugation

    Pages 61-73

    Harding, Stephen E.

Buy this book

eBook $84.99
price for USA in USD (gross)
  • ISBN 978-1-59259-509-9
  • Digitally watermarked, DRM-free
  • Included format: EPUB, PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $179.99
price for USA in USD
  • ISBN 978-0-89603-232-3
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $109.99
price for USA in USD
  • ISBN 978-1-4899-4001-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Microscopy, Optical Spectroscopy, and Macroscopic Techniques
Authors
Series Title
Methods in Molecular Biology
Series Volume
22
Copyright
1994
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-509-9
DOI
10.1385/0896032329
Hardcover ISBN
978-0-89603-232-3
Softcover ISBN
978-1-4899-4001-8
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XI, 251
Topics