Methods in Molecular Biology

Animal Cell Culture

Editors: Pollard, Jeffrey W., Walker, John M. (Eds.)

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About this book

For many years I performed tissue culture in large scientific insti- tions that had a great deal of infrastructure. When I set up a tissue l- oratory outside such an infrastructure, however, I found there was a shortage of easily accessible information about the basic needs, reagents, and techniques for establishing such a facility. Much had to be done by trial and error or gleaned from originalpapers. Consequently, I felt that a methods book covering a wide variety of techniques from basic culture to the most sophisticated cell analysis would be a very valuable addition to the scientific literature. In the interim, several useful books (listed in Chapter I of this volume) did appear, but none entirely fitted the bill and some are now somewhat dated. Then, in 1984, the first of the Methods in Molecular Biology volumes from Humana Press was published with its step-by-step recipe approach. This format appealed to me, and so I c- tacted John Walker, the series editor, about including cell culture in this series. The result was that we embarked upon a single volume covering both plant and animal cell culture. Such was the richness of the material that this project soon divided itself into separate volumes on animal cell (Volume 5) and plant cell (Volume 6) culture. In this volume (Volume 5), therefore, we have aimed to describe a variety of basic techniques and culture conditions for a range of cell types.

Reviews

"The clear and well presented protocols... are an invaluable asset....Well-presented and essential." -SGM Quarterly

"The book contains 40 chapters written by a panel of authors who are all experts in their field...A wide range of topics relevant to the epidemiology, pathogenesis, clinical features and treatment of meningococcal disease are covered...this book is a valuable addition to the limited literature on meningococci and meningococcal infection. The methodological sections are detailed, carefully written and should allow technology transfer to additional laboratories." -Molecular Biotechnology

Table of contents (49 chapters)

  • Basic Cell Culture

    Pollard, Jeffrey W.

    Pages 1-12

  • Establishment, Maintenance, and Cloning of Human Primary Cell Strains

    Jones, Gareth E.

    Pages 13-23

  • Aging of Cultured Human Skin Fibroblasts

    Harley, Calvin B.

    Pages 25-32

  • Separation and Maintenance of Primary T and B Lymphocytes

    Kinchington, Derek (et al.)

    Pages 33-41

  • Establishment of Lymphoblastoid Cell Lines

    Doyle, A.

    Pages 43-47

Buy this book

eBook $139.00
price for USA in USD (gross)
  • ISBN 978-1-59259-492-4
  • Digitally watermarked, DRM-free
  • Included format: PDF, EPUB
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $249.99
price for USA in USD
  • ISBN 978-0-89603-150-0
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $179.99
price for USA in USD
  • ISBN 978-1-4899-4385-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Animal Cell Culture
Editors
  • Jeffrey W. Pollard
  • John M. Walker
Series Title
Methods in Molecular Biology
Series Volume
5
Copyright
1990
Publisher
Humana Press
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-59259-492-4
DOI
10.1385/0896031500
Hardcover ISBN
978-0-89603-150-0
Softcover ISBN
978-1-4899-4385-9
Series ISSN
1064-3745
Edition Number
1
Number of Pages
XIV, 713
Topics