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Methods of Protein Microcharacterization

A Practical Handbook

  • Book
  • © 1986

Overview

Editors:
  1. John E. Shively

    1. Beckman Research Institute of the City of Hope, Duarte

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Part of the book series: Biological Methods (BM)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xix
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  3. Microamino Acid Analysis

    1. Front Matter

      Pages 103-103
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    2. Amino Acid Analysis With Postcolumn Fluorescent Derivatization

      • Yu-Ching E. Pan, Stanley Stein
      Pages 105-119

    3. Amino Acid Analysis by o-Phthaldialdehyde Precolumn Derivatization and Reverse-Phase HPLC

      • Barry Nelson Jones
      Pages 121-151

  4. Amino Terminal Analysis

    1. Front Matter

      Pages 153-153
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    2. Manual Edman Sequencing System

      • George E. Tarr
      Pages 155-194

    3. Comparison of the Spinning Cup and Gas-Phase Instruments

      • John E. Shively
      Pages 195-222

    4. Gas-Phase Protein/Peptide Sequencer

      • Michael W. Hunkapiller, Kristina Granlund-Moyer, Norman W. Whiteley
      Pages 223-247

    5. Design of a Multipurpose Sequencer

      • Brigitte Wittmann-Liebold
      Pages 249-277

    6. Solid-Phase Methods in Protein Microsequence Analysis

      • James J. L’Italien
      Pages 279-314

    7. Analysis of Phenylthiohydantoin Amino Acids by HPLC

      • Michael W. Hunkapiller, Kristina Granlund-Moyer, Norman W. Whiteley
      Pages 315-327

    8. Purification and Preparation of Chemicals for Microsequencing With the Edman Degradation

      • Michael W. Hunkapiller, Chin-Sook Kim
      Pages 329-334

  5. Carboxyl-Terminal Analysis

    1. Front Matter

      Pages 335-335
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    2. Microsequence Analysis by Enzymatic Methods

      • Barry Nelson Jones
      Pages 337-361

    3. COOH-Terminal Analysis of Polypeptides by Chemical and Chromatographic Methods

      • Joachim Spiess
      Pages 363-377

  6. Mass Spectrometric Analysis of Polypeptides

    1. Front Matter

      Pages 379-379
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Keywords

Editors and Affiliations

  • Beckman Research Institute of the City of Hope, Duarte

    John E. Shively

Bibliographic Information

  • Book Title: Methods of Protein Microcharacterization

  • Book Subtitle: A Practical Handbook

  • Editors: John E. Shively

  • Series Title: Biological Methods

  • DOI: https://doi.org/10.1007/978-1-59259-436-8

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Book Archive

  • Copyright Information: Springer Science+Business Media LLC 1986

  • Hardcover ISBN: 978-0-89603-090-9Published: 08 July 1986

  • Softcover ISBN: 978-1-4899-4132-9Published: 27 November 2013

  • eBook ISBN: 978-1-59259-436-8Published: 22 February 2008

  • Edition Number: 1

  • Number of Pages: XIX, 456

  • Topics: Biochemistry, general

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