Methods in Protein Sequence Analysis

1. Front Matter

   Pages i-xxvii

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2. Conference Lecture

   1. Front Matter

      Pages xxvii-xxvii

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   2. An Anecdotal Account of the History of Peptide Stepwise Degradation Procedures

      • Russell F. Doolittle

      Pages 1-24

3. Overview

   1. Front Matter

      Pages 25-25

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   2. An Evaluation of the Current Status of Protein Sequencing

      • Brigitte Wittmann-Liebold

      Pages 27-63

4. Strategy

   1. Front Matter

      Pages 65-65

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   2. Strategic Approaches to Sequence Analysis

      • Kenneth A. Walsh

      Pages 67-73

5. Improvements in Instrumentation and the Chemistry of Isothiocyanate Degradation

   1. Front Matter

      Pages 75-75

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   2. A New Protein Microsequenator Using Gas Phase Edman Reagents

      • Michael W. Hunkapiller, Rodney M. Hewick, William J. Dreyer, Leroy E. Hood

      Pages 77-90

   3. Towards Longer Degradations on a Sequenator

      • Gerhard Frank

      Pages 91-99

   4. The Nature of Non-Specific Peptide Bond Cleavage During the Isothiocyanate Degradation of Proteins

      • Wolf F. Brandt, Agnes Henschen, Claus Von Holt

      Pages 101-110

6. Microsequencing

   1. Front Matter

      Pages 111-111

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   2. Isolation and Sequence Determination of Polypeptides at the Picomole Level

      • J. Y. Chang, R. Knecht, D. G. Braun

      Pages 113-118

   3. Methodologies in the Microsequencing

      • Ajit S. Bhown, John E. Mole, J. Claude Bennett

      Pages 119-130

   4. Micro-Sequence Analysis of Ovine Hypothalamic Corticotropin Releasing Factor

      • Joachim Spiess, Jean Rivier, Catherine Rivier, Wylie Vale

      Pages 131-138

   5. Identification of Internal Residues of Lactose Permease of Escherichia Coli by Radiolabel Sequencing of Peptide Mixtures

      • Konrad Beyreuther, Barbara Bieseler, Ruth Ehring, Benno Müller-Hill

      Pages 139-148

   6. The Application of Monoclonal Antibodies to the Microsequencing of Proteins

      • Lawrence K. Duffy, Alexander Kurosky

      Pages 149-156

7. Solid Phase Sequencing

   1. Front Matter

      Pages 157-157

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   2. Sensitivity Enhancement in Solid-Phase Protein Sequence Analysis: An Examination of Methodology

      • Marcus J. Horn, Alex G. Bonner

      Pages 159-171

   3. Progress in Solid-Phase Sequencing: Extended Degradations of Peptides and Proteins

      • Werner Machleidt, Helmut Hofner

      Pages 173-180

Methods in Protein Sequence Analysis contains an intensely prac­ tical account of all the new methodology available to scientists carrying out protein and peptide sequencing studies. Many of the striking advances in fields as diverse as immunology, cell motility, and neurochemistry have in fact been fueled by our ever more powerful ability to determine the sequences and structures of key proteins and peptides. It is our hope that the rich array of tech­ niques and methods for sequencing proteins discussed in this volume-methods that generate much of the information crucial to progress in modern biology-will now become accessible to all who can benefit from them. The papers of the present volume constitute the Proceedings of the IVth International Conference on Methods in Protein Se­ quence Analysis, which was held at Brookhaven National Labo­ ratory, Upton, NY, September 21-25, 1981. It was the most recent in a series of biennial conferences, the previous one having been held in Heidelberg, GFR, in 1979. The series was originated by Richard Laursen, and initially dealt with one aspect of the field, solid-phase sequencing. The scope of the meeting was very broad and among the many aspects of protein sequencing discussed were: instrumentation, strategy, chemicals, mass spectrometry, cleavage of proteins and separation of peptides, and solid, liquid, manual, and even "gas­ phase" sequencing.

• ATP
• ATP-Synthase
• Amino acid
• DNA
• Methodologie
• Nucleotide
• RNA
• enzymes
• primary structure
• protein sequence
• protein structure
• proteins

• Brookhaven National Laboratory, Upton, USA

  Marshall Elzinga

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