Save 40% on books and eBooks in Engineering & Materials Science or in Social & Behavioral Sciences!

Perspectives in Antisense Science

Manual of Antisense Methodology

Editors: Hartmann, Gunther, Endres, Stefan (Eds.)

Free Preview

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4615-5067-9
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-0-7923-8539-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4613-7304-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
About this book

In the past few years, antisense methodology has moved from in vitro studies to in vivo studies and first human trials. While the basic concept of antisense technology is simple, the methodological problems associated with its use are numerous and complex. Antisense- based methods have proven to be a field of research where careful attention to experimental protocols and appropriate controls is necessary.
The Manual of Antisense Methodology emphasizes the application of antisense oligonucleotides, and is a guide for the identification of antisense and non-antisense effects in different experimental settings. The work is organized into three sections: antisense application in vitro, antisense application in vivo (animal models) and finally, clinical antisense studies. Where at all possible, the methods are described in sufficient detail to allow reproduction of a given experiment.
The Manual of Antisense Methodology will be of interest to researchers in immunology, cancer research, pharmacology and internal medicine; and physicians conducting clinical studies in these fields.

About the authors

Gunther Hartmann, M.D. is a clinical and research fellow at the Division of Clinical Pharmacology, University of Munich. Stefan Endres, M.D. is an internist with subspecialties in gastroenterology and intensive care medicine. He is Chief of the Division of Clinical Pharmacology of the medical faculty at the University of Munich.

Table of contents (11 chapters)

Table of contents (11 chapters)
  • Chemical Synthesis and Purification of Phosphorothioate Antisense Oligonucleotides

    Pages 3-23

    Sanghvi, Yogesh S. (et al.)

  • How to Choose Optimal Antisense Targets in an mRNA

    Pages 25-38

    Schu, Bernhard (et al.)

  • How to Characterize and Improve Oligonucleotide Uptake into Leukocytes

    Pages 41-56

    Bidlingmaier, Martin (et al.)

  • Strategies for Targeted Uptake of Antisense Oligonucleotides in Hepatocytes

    Pages 57-77

    Offensperger, Wolf-Bernhard (et al.)

  • How to Exclude Immunostimmulatory and Other Nonantisense Effects of Antisense Oligonucleotides

    Pages 79-89

    Krieg, Arthur M.

Buy this book

eBook $109.00
price for USA in USD (gross)
  • ISBN 978-1-4615-5067-9
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $219.99
price for USA in USD
  • ISBN 978-0-7923-8539-4
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $149.99
price for USA in USD
  • ISBN 978-1-4613-7304-9
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Loading...

Recommended for you

Loading...

Bibliographic Information

Bibliographic Information
Book Title
Manual of Antisense Methodology
Editors
  • Gunther Hartmann
  • Stefan Endres
Series Title
Perspectives in Antisense Science
Series Volume
4
Copyright
1999
Publisher
Springer US
Copyright Holder
Springer Science+Business Media New York
eBook ISBN
978-1-4615-5067-9
DOI
10.1007/978-1-4615-5067-9
Hardcover ISBN
978-0-7923-8539-4
Softcover ISBN
978-1-4613-7304-9
Series ISSN
1566-0389
Edition Number
1
Number of Pages
XIII, 205
Topics