Animal Cell Technology: Products from Cells, Cells as Products

Improvement and Induction of High Productivity

1. Front Matter

   Pages 1-1

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2. Recombinant Pharmaceutical Protein Overexpression in an IRF-1 Proliferation Controlled Production System

   • C. Geserick, K. Schroeder, H. Bonarius, L. Kongerslev, P. Schlenke, H. Hauser et al.

   Pages 3-9

3. The Real Meaning of High Expression

   • S. I. Grammatikos, K. Bergemann, W. Wwrz, I. Brax, R. Bux, P. Eberhardt et al.

   Pages 11-17

4. A Novel Mammalian Gene Regulation System Responsive to Streptogramin Antibiotics

   • M. Fussenegger, B. Von Stockar, C. Fux, M. Rimann, R. Morris, C.J. Thompson et al.

   Pages 19-22

5. Feedback Control of Redox Potential in Hybridoma Cell Culture

   • A. Meneses, A. Gomez, O.T. Ramirez

   Pages 23-29

6. Genetic Manipulation of the Protein Synthetic Capacity of Mammalian Cells

   • Michèle Heaton, John Birch, Alison Hovey, Robert Kallmeier, Chris Proud, David James

   Pages 31-33

7. Retinoic Acid Enhances Monoclonal Antibody Production of Human-Human Hybridoma BD9

   • Y. Inoue, M. Fujisawa, M. Shoji, S. Hashizume, Y. Katakuma, S. Shirahata

   Pages 35-37

8. Extracellular Adenosine 5’ Monophosphate Controls Proliferation and Stimulates Protein Production of rCHO Cells

   • Hendrik P.J. Bonarius, Karen Hansen, Leif Kongerslev

   Pages 39-41

9. The Mode of Actions of Lysozyme as an Immunoglobul in Production Stimulating Factor (IPSF)

   • T. Sugahara, F. Murakami, T. Sasaki

   Pages 43-45

10. A Dissection of Recombinant Protein Expression in CHO Cells Indicating Protein-Specific, not Cells Specific, Limitation

    • K. Altobello, Y. Chung, J. Iovino, C. Luchette, S. Overton, Mark Cunningham

    Pages 47-49

11. Effect of Bioreactor Process Control Parameters on Apoptosis and Monoclonal Antibody Production During the Protein-Free Fed-Batch Culture of a Murine Myeloma

    • Emda Moran, Steve Mcgowan, Niamh Reynolds, Claire Wilson

    Pages 51-53

12. Adenovirus Vector Production in 293 Fed-Batch Cultyres

    • Kathy Wong, Maria Jesus Guardia, Stanford Lee, Weishou Hu

    Pages 55-57

13. Influence of BCL-2 Over-Expression on NS0 and CHO Culture Viability and Chimeric Antibody Productivity

    • B.T. Tey, R.P. Singh, M. Al-Rubeai

    Pages 59-61

14. A Regulatable Selective System Facilitating Isolation of High Expression Mammalian Cells

    • Kiichiro Teruya, Ying-Pei Zhang, Yoshinori Katakura, Sanetaka Shirahata

    Pages 63-65

15. Comparative Analysis of IRES Efficiency of Dicistronic Expression Vectors in Primary Cells and Permanent Cell Line

    • Lorin Schumacher, Paula M. Alves, Manfred Wirth

    Pages 67-69

16. Use of Gel Microdrops Selection System for Recombinant Cells

    • Karen Hansen, Charlotte Bertelsen, Leif Kongerslev

    Pages 71-73

17. Bovine Serum-Albumin with Grafted RGD Tailed Cyclic Peptudes as an Engineered Proadhesive Protein

    • Janique Dewelle, Dominiqe Delforge, Sabine Pirotton, Jose Remacle, Martine Raes

    Pages 75-77

18. Matrix Attachment Regions in Stable CHO Cell Line Development

    • Monique M. Zahn, M. Kobr, N. Mermod

    Pages 79-81

19. High Cell Density Cultivation of Hybridoma Cells: Spin Filter vs Immobilized Culture

    • H. Heine, M. Biselli, C. Wandrey

    Pages 83-85