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Developments in Molecular and Cellular Biochemistry

Novel Methods in Molecular and Cellular Biochemistry of Muscle

Editors: Pierce, Grant, Claycomb, William C. (Eds.)

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About this book

Experimental techniques are the life blood of science. The better the methodology is, the more reliable and accurate the results will be. Ultimately, this will lead to a clearer interpretation of those results and firmer conclusions from any set of experiments. Experimental methodology in the area of cardiovascular biochemistry and molecular biology has advanced considerably in the last decade. Because of these factors, it was thought that a focused issue of Molecular and Cellular Biochemistry dedicated to the novel, latest technological advances in the field was warranted. We must thank Dr Naranjan S. Dhalla, Editor-in-Chief of Molecular and Cellular Biochemistry, for his willingness to publish an issue with such a focus. We have attracted some of the leaders in the field of cardiovascular biology to submit articles describing some of the most novel, significant techniques currently in use in their laboratories. The purpose of the manuscripts was not to describe the recent experimental findings from each laboratory as is done in most conventional manuscripts. Instead, the purpose of the articles found within this focused volume of Molecular and Cellular Biochemistry was to describe how the technique is performed on the laboratory bench so that others less familiar with the technique may be able to use it in their own labs. The subjects described in this volume can be generally subdivided into three categories: molecular biology, cell biology and basic biochemistry. The methods cover wide areas including various DNA and RNA expression technologies, transfection techniques, quantification of ion flux movement, measurements of lipid metabolism, advances in the culture of specific cardiovascular cell populations, and the use of confocal microscopy to examine cell structure and function. We thank all of the authors who have contributed so much of their time and efforts and, most importantly, shared the `secrets' of these valuable techniques with the rest of the cardiovascular research community.

Table of contents (22 chapters)

Table of contents (22 chapters)
  • DNA transfer into vascular smooth muscle using fusigenic Sendai virus (HJV)-liposomes

    Pages 3-12

    Mann, Michael J. (et al.)

  • Adenovirus mediated — gene transfer into cardiomyocytes

    Pages 13-21

    Kirshenbaum, Lorrie A.

  • Retroviral techniques for studying organogenesis with a focus on heart development

    Pages 23-35

    Hyer, Jeanette (et al.)

  • Novel methods for adenovirus-mediated gene transfer to blood vessels in vivo

    Pages 37-46

    Ooboshi, Hiroaki (et al.)

  • Use of a hammerhead ribozyme with cationic liposomes to reduce leukocyte type 12-lipoxygenase expression in vascular smooth muscle

    Pages 47-57

    Gu, Jia-Li (et al.)

Buy this book

eBook $219.00
price for USA in USD (gross)
  • ISBN 978-1-4615-6353-2
  • Digitally watermarked, DRM-free
  • Included format: PDF
  • ebooks can be used on all reading devices
  • Immediate eBook download after purchase
Hardcover $399.99
price for USA in USD
  • ISBN 978-0-7923-4387-5
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
Softcover $279.99
price for USA in USD
  • ISBN 978-1-4613-7918-8
  • Free shipping for individuals worldwide
  • Usually dispatched within 3 to 5 business days.
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Bibliographic Information

Bibliographic Information
Book Title
Novel Methods in Molecular and Cellular Biochemistry of Muscle
Editors
  • Grant Pierce
  • William C. Claycomb
Series Title
Developments in Molecular and Cellular Biochemistry
Series Volume
20
Copyright
1997
Publisher
Springer US
Copyright Holder
Springer Science+Business Media Dordrecht
eBook ISBN
978-1-4615-6353-2
DOI
10.1007/978-1-4615-6353-2
Hardcover ISBN
978-0-7923-4387-5
Softcover ISBN
978-1-4613-7918-8
Edition Number
1
Number of Pages
V, 241
Topics