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Structural Biology

Practical NMR Applications

  • Textbook
  • © 2005

Overview

Authors:
  1. Quincy Teng

    1. Department of Chemistry, University of Georgia, Athens

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  • NMR spectroscopy laid out in a straight forward manner

  • Detailed descriptions including equipment and hardware components

  • Useful practical aspects of NMR processes and techniques

  • Comprehensive coverage of NMR sample preparation with several routine strategies for preparing samples for macromolecules as well as complexes

  • Includes supplementary material: sn.pub/extras

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Table of contents (8 chapters)

  1. Front Matter

    Pages i-xii
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  2. Basic Principles of NMR

    Pages 1-55

  3. Instrumentation

    Pages 57-88

  4. NMR Sample Preparation

    Pages 89-100

  5. Practical Aspects

    Pages 101-148

  6. Multidimensional Heteronuclear NMR Experiments

    Pages 149-189

  7. Studies of Small Biological Molecules

    Pages 191-210

  8. Protein Structure Determination from NMR Data

    Pages 211-243

  9. Protein Dynamics

    Pages 245-262

  10. Back Matter

    Pages 263-295
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Keywords

About this book

Over the years since NMR was first applied to solve problems in structural biology, it has undergonedramaticdevelopmentsinbothNMRinstrumenthardwareandmethodology. While it is established that NMR is one of the most powerful tools for understanding biological p- cesses at the atomic level, it has become increasingly difficult for authors and instructors to make valid decisions concerning the content and level for a graduate course of NMR in str- turalbiology. BecausemanyofthedetailsinpracticalNMRarenotdocumentedsystematically, students entering the field have to learn the experiments and methods through communication with other experienced students or experts. Often such a learning process is incomplete and unsystematic. This book is meant to be not only a textbook, but also a handbook for those who routinely use NMR to study various biological systems. Thus, the book is organized with experimentalists in mind, whether they are instructors or students. For those who have a little or no background in NMR structural biology, it is hoped that this book will provide sufficient perspective and insight. Those who are already experienced in NMR research may find new information or different methods that are useful to their research. Because understanding fundamental principles and concepts of NMR spectroscopy is essential for the application of NMR methods to research projects, the book begins with an introduction to basic NMR principles. While detailed mathematics and quantum mechanics dealing with NMR theory have been addressed in several well-known NMR books, Chapter 1 illustrates some of the fundamental principles and concepts of NMR spectroscopy in a more descriptiveandstraightforwardmanner.

Reviews

"This is the first totally through book on the subject. It covers theory, the instrument, how to run an experiment, the latest in pulse and structure determination methods. The best part is that it’s written as a textbook with questions and goal at the beginning of each section and homework or exam problems. It is ideal for a graduate or advanced undergraduate course on biological NMR."
- Lawrence J. Berliner, University of Denver, Denver, CO, USA

"While NMR treatises abound, there exists no single text that provides both the information necessary to understand the experiments and processes, as well as the practical details for their successful implementation. This considerable gap in the literature is now happily bridged by Quincy Teng’s timely contribution."
- Gerd N. La Mar, University of California, Davis, CA, USA

Authors and Affiliations

  • Department of Chemistry, University of Georgia, Athens

    Quincy Teng

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