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Confocal Microscopy for Biologists

  • Textbook
  • © 2004

Overview

Authors:
  1. Alan R. Hibbs

    1. BIOCON, Melbourne, Australia

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  • Explores the basic concepts of microscopy, fluorescence, digital imaging
  • Outlines the principles of confocal microscopy
  • Teaches readerds to take full advantage of the confocal microscopes now available

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Table of contents (15 chapters)

  1. Front Matter

    Pages i-xiii
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  2. What is Confocal Microscopy?

    • Alan R. Hibbs
    Pages 1-29

  3. Understanding Microscopy

    • Alan R. Hibbs
    Pages 31-63

  4. Confocal Microscopy Hardware

    • Alan R. Hibbs
    Pages 65-99

  5. Image Collection

    • Alan R. Hibbs
    Pages 101-144

  6. Digital Images in Microscopy

    • Alan R. Hibbs
    Pages 145-162

  7. Imaging Software

    • Alan R. Hibbs
    Pages 163-176

  8. Presentation and Publication

    • Alan R. Hibbs
    Pages 177-185

  9. What is Fluorescence?

    • Alan R. Hibbs
    Pages 187-200

  10. Fluorescent Probes

    • Alan R. Hibbs
    Pages 201-238

  11. Confocal Microscopy Techniques

    • Alan R. Hibbs
    Pages 239-257

  12. Fluorescence Immunolabelling

    • Alan R. Hibbs
    Pages 259-277

  13. Imaging Live Cells

    • Alan R. Hibbs
    Pages 279-323

  14. The Internet

    • Alan R. Hibbs
    Pages 325-333

  15. Technical Supplies

    • Alan R. Hibbs
    Pages 335-346

  16. Further Reading

    • Alan R. Hibbs
    Pages 347-353

  17. Back Matter

    Pages 355-467
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Keywords

About this book

There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.

Reviews

From the reviews:

"My overall impression of this book is that it offers an excellent compilation of high-standard reviews on basic concepts of microscopy and the principles of confocal microscopy. … all of the illustrations and graphs contained in the book are well-designed and concise. In summary, this is an outstanding book. For beginners and advanced users of confocal microscopy, from young scientists to professionals, this is a worthwhile read. The text has enormous value for biologists … . I enjoyed reading this book … ." (Rafael Luján, Journal of Chemical Neuroanatomy, Vol. 30, 2005)

Authors and Affiliations

  • BIOCON, Melbourne, Australia

    Alan R. Hibbs

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