- Summarizes transgenesis techniques in ascidians and up-to-date concrete research using those techniques
- Enables readers to quickly access and compare techniques at a glance
- Helps readers to apply the strategies contained here to their own studies
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- About this book
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This book comprehensively describes the transgenesis techniques and applied experimental methods in ascidians including enthusiastically developed original devices in addition to concrete examples of developmental biology studies.
Ascidians have been one of the most important model animals in developmental biology for studying molecular and cellular processes underlying formation of the chordate body plan. Transgenic techniques such as microinjection, electropolation, cis-element analysis and application, and TALENs and CRISPR/Cas9 have been developed in ascidians for more than 20 years, and now many applied methods, some of which are unique in ascidians, have been accumulated. Those extensive technological innovations, such as cell isolation, cell labeling, germ-line transformation, marker transgenic lines, and the experimental systems for studying notochord formation and nervous system, are exceptional particularly in marine invertebrates.
This book is useful for ascidian researchers to quickly access the techniques in which they are interested as well as to compare each technology to become familiar with specialized tips, and for biologists of other organisms to learn the unique techniques and ingenious attempts specific to ascidians. Providing detailed and easily understandable descriptions of techniques, the book will inspire ascidian specialists to improve their techniques, encourage anyone wanting to begin studying ascidians, and enable readers to immediately apply the techniques to the organisms they research.
- About the authors
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Yasunori Sasakura started his research career in Dr. Noriyuki Satoh’s laboratory in 1996. His Ph.D. thesis was about the localization of mRNAs in eggs of the ascidian Halocynthia roretzi. He then focused on developing the genetic approaches in the ascidian Ciona intestinalis. The reason is that he thought that genetics would be necessary to elucidate molecular mechanisms of maternally stored substances. He introduced transposon-mediated germ cell transgenesis and mutagenesis in this ascidian. From then on he brought many technical innovations in the study of Ciona with transgenic techniques. For example, his group has introduced transgenesis-mediated tissue/cell tracing with the photoconvertible protein Kaede, transposon-based specific knockdown of maternally transcribed RNAs, the optogenetic approach to understand neuronal functions, and genome editing techniques in Ciona with TALENs and CRISPR/Cas9.
- Table of contents (16 chapters)
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Introduction
Pages 1-3
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Microinjection of Exogenous Nucleic Acids into Eggs: Ciona Species
Pages 5-13
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Practical Guide for Ascidian Microinjection: Phallusia mammillata
Pages 15-24
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Microinjection of Exogenous DNA into Eggs of Halocynthia roretzi
Pages 25-35
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Electroporation in Ascidians: History, Theory and Protocols
Pages 37-48
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Table of contents (16 chapters)
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Bibliographic Information
- Bibliographic Information
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- Book Title
- Transgenic Ascidians
- Editors
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- Yasunori Sasakura
- Series Title
- Advances in Experimental Medicine and Biology
- Series Volume
- 1029
- Copyright
- 2018
- Publisher
- Springer Singapore
- Copyright Holder
- Springer Nature Singapore Pte Ltd.
- eBook ISBN
- 978-981-10-7545-2
- DOI
- 10.1007/978-981-10-7545-2
- Hardcover ISBN
- 978-981-10-7544-5
- Softcover ISBN
- 978-981-13-3965-3
- Series ISSN
- 0065-2598
- Edition Number
- 1
- Number of Pages
- X, 201
- Topics
